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Sample GSM213702 Query DataSets for GSM213702
Status Public on Aug 10, 2007
Title LPS_pre_LPS_restimulation_rep_A
Sample type RNA
Source name macrophages with 1_1 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
VALUE rma normalized value (linear scale)

Data table
1415670_at 755.05 P
1415671_at 2867.39 P
1415672_at 2285.75 P
1415673_at 102.32 P
1415674_a_at 1285.32 P
1415675_at 599.49 P
1415676_a_at 3151.04 P
1415677_at 1206.81 P
1415678_at 1431.2 P
1415679_at 2705.66 P
1415680_at 855.49 P
1415681_at 769.94 P
1415682_at 280.44 P
1415683_at 2825.66 P
1415684_at 777.29 P
1415685_at 300.51 P
1415686_at 1751.23 P
1415687_a_at 9451.53 P
1415688_at 1024.92 P
1415689_s_at 333.01 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.

Supplementary file Size Download File type/resource
GSM213702.CEL.gz 6.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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