|
| Status |
Public on Jun 30, 2008 |
| Title |
C10 congenic_430B_hippocamous_pool3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
Interval-specific congenic strain of Chromosome 10 (C10 congenic) mice were generated and maintained on the A/J background after at least 10 backcrosses as heterozygotes (B6/A) for the chr. 10 region, male , adult hippocampus, 3 hippocampi /pool
|
| Biomaterial provider |
.
|
| Growth protocol |
All mice of the same strain and gender were raised in groups of four with food (Teklad 7002; 6%fat; Harlan Teklad, Madison, Wi.) and water freely available. All experiments followed the NIH Guide for the Care and Use of Laboratory Animals and received Institutional Animal Care and Use approval.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s manual. RNA was treated with DNA-freeTM (Ambion, Austin, Tx.) to remove residual contaminating DNA.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
C10 congenic_430B_hippocamous_pool3
|
| Data processing |
The raw image files of Affymetrix microarrays were analyzed and normalized using the Probe Logarithmic Intensity Error estimation (PLIER) algorithm in the ArrayAssist Expression software package (Stratagene, La Jolla, Ca). The software performed a two-step analysis: one for present/absent calls by MAS5, and the other for data generation by PLIER.
|
| |
|
| Submission date |
Jul 30, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sanghyeon Kim |
| E-mail(s) |
Sanghyeon.Kim@UTSouthwestern.edu
|
| Organization name |
UT Southwestern medical center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9070 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE8641 |
Rnf41 is associated with anxiety like behavior, major depression and beta carboline induced seizure |
|
