|
Status |
Public on Mar 01, 2017 |
Title |
BreastTumor_COCA-61-A-T [miRNA] |
Sample type |
RNA |
|
|
Source name |
Breast primary tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue type: Breast tumor tissue hospital id: 1 estrogen receptor (er) status: pos human epidermal growth factor receptor 2 (her2) status: NA histological grade: 2 pam50 subtype: Normal
|
Treatment protocol |
Fresh frozen in liquid nitrogen
|
Growth protocol |
Human primary tumors
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol, following manufactors protocol
|
Label |
Cy3
|
Label protocol |
Agilent miRNA Labeling Reagent and Hybridization Kit, Protocol v.2.3.
|
|
|
Hybridization protocol |
Agilent miRNA Labeling Reagent and Hybridization Kit, Protocol v.2.3.
|
Scan protocol |
Scanning on Agilent scanner G2565A and Feature Extraction (FE) v10.7.3.1 was used to extract signals
|
Data processing |
Signals were extracted using Feature Extraction v10.7.3.1 and the subsequent data processing was performed using the GeneSpring software v11.0 (Agilent Technologies). In brief, the miRNA signal intensities were log2-transformed and normalized to the 90th percentile, and miRNAs that were detected in less than 10% of the samples were excluded. This resulted in 421 unique mature miRNAs.
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|
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Submission date |
May 02, 2016 |
Last update date |
Mar 01, 2017 |
Contact name |
Miriam Ragle Aure |
E-mail(s) |
mirrag@rr-research.no
|
Phone |
+47 22781363
|
Organization name |
Oslo University Hospital Radiumhospitalet
|
Department |
Dept. of Cancer Genetics
|
Street address |
Ullernchauséen 70
|
City |
Oslo |
ZIP/Postal code |
0310 |
Country |
Norway |
|
|
Platform ID |
GPL10656 |
Series (2) |
GSE81000 |
Integrative clustering reveals a novel split in the luminal A subtype of breast cancer with impact on outcome |
GSE81002 |
Integrative clustering reveals a novel split in the luminal A subtype of breast cancer with impact on outcome |
|