|
| Status |
Public on Jan 06, 2017 |
| Title |
iLin28b_spleen |
| Sample type |
SRA |
| |
|
| Source name |
splenic CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
exogenous expression: Human Lin28b
tissue: Spleen
|
| Treatment protocol |
Bone marrow cells from gBT-I mice, either WT or expressing inducible Lin28b, was infected in an adult mouse whose bone marrow had been irradiated. Mice then received doxycycline in their drinking water to induce Lin28b. Cells were used 8 weeks after reconstitution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol from purified cell populations
NEBNext Multiplex Small RNA Library Prep Set for Illumina
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Adapters were trimmed using Cutadapt v1.8.3
Trimmed reads were mapped and quantified to miRNAs using Mirdeep2 and Bowtie (miRBase version 21)
Genome_build: mm9
Supplementary_files_format_and_content: Mirdeep2 output flle; contains miRNA, number reads mapped to the miRNA across samples, precursor-miRNA, total number reads, number reads per sample, and normalized (reads per million) reads per sample
|
| |
|
| Submission date |
May 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Grimson |
| Organization name |
Cornell University
|
| Department |
Molecular Biology & Genetics
|
| Street address |
445 Biotechnology Building
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE80597 |
Fetal and adult progenitors give rise to unique populations of CD8+ T cells |
|
| Relations |
| BioSample |
SAMN04942643 |
| SRA |
SRX1742377 |
