Rats were fed with Harlan Teklad Rodent diet, 4% mash diet (control)
Extracted molecule
total RNA
Extraction protocol
Stat-60 extraction
Label
biotin
Label protocol
Enzo labeling kit
Hybridization protocol
Following fragmentation, 20ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using the Affymetrix 7G GeneChip Scanner .
Description
Gene expression data from livers of rats that were not treated with chemicals
Data processing
The data were analyzed with Bioconductor using GC-RMA analysis.
