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Sample GSM22719 Query DataSets for GSM22719
Status Public on May 21, 2004
Title A chip: time 30 min Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.74E-01 0 269.019 226.519 215.707 181.626 166.5 111 125 88
2 1.18E-01 0 268.584 219.19 227.334 187.248 153 115.5 115.5 99.5
3 3.58E-01 0 776.06 252.591 571.605 171.03 636 149.5 417 96
4 3.75E-01 0 260.216 211.716 225.011 225.376 164 105 117 122
5 -5.56E-01 0 1284.87 254.644 1583.75 240.159 1064 153 1313 117.5
6 -2.96E-02 0 356.224 222.776 337.914 195.612 228 115 193 105
7 1.61E-01 0 270.913 219.629 239.927 199.223 153 107 124 105
8 -3.80E-01 0 243.552 243.826 218.186 184.519 136 136 120 97.5
9 -4.17E-01 0 1025.48 252.534 1199.43 233.301 857 140 903 133
10 2.98E-01 0 262.368 247.22 200.566 184.698 151 121 111 102
11 -7.44E-01 0 360.96 286.97 420.845 181.312 224 157.5 276 96
12 4.38E-01 0 479.807 266.667 326.993 208.706 323 168 206 105
13 -6.21E-01 0 1292.31 261.837 1630.58 198.125 1007 165.5 1223 105.5
14 7.93E-01 0 274.035 225.33 195.273 206.299 170 119 104 117
15 9.36E-01 0 235.398 239.701 188.989 240.602 140.5 130.5 108.5 130
16 -4.47E-01 0 264.005 263.852 238.118 201.399 152 144.5 126 96
17 2.13E-01 0 373.223 217.577 317.209 206.428 234.5 125.5 175 116
18 -2.24E-02 0 805.186 209.401 787.854 206.556 618 113 585 123
19 4.48E-01 0 268.922 197.979 198.355 216.057 149 118.5 103 104
20 -3.84E-01 0 254.053 234.174 217.783 203.348 150 153.5 110 103.5

Total number of rows: 16928

Table truncated, full table size 1089 Kbytes.




Supplementary data files not provided

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