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Sample GSM22726 Query DataSets for GSM22726
Status Public on May 21, 2004
Title A chip: time 1 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -3.64E-01 0 301.119 266.189 261.976 194.118 163.5 151 147 105.5
2 -1.43E-01 0 324.752 259.833 280.605 243.331 195 144 166 114
3 8.12E-01 0 3692.09 251.026 1642.35 220.811 3505 134.5 1407 113.5
4 -1.86E-01 0 277.989 216.376 233.791 182.533 154 131 120 98
5 -3.03E-01 0 4831.23 266.48 3742.51 241.523 4031 169.5 3323 148
6 6.29E-01 0 661.969 256.982 453.505 215.11 511 145 328 115
7 -5.67E-02 0 334.226 228.324 291.148 169.508 207 120.5 159 99
8 -5.43E-01 0 236.598 250.093 198.739 172.993 118 139 101 85
9 -4.46E-01 0 1361.13 212.211 1451.81 189.767 1037 109.5 994 94.5
10 -2.70E-01 0 218.259 204.27 202.748 203.626 108 107 106 95
11 -5.98E-01 0 330.579 189.771 422.818 178.19 206 93 264 98
12 1.05E+00 0 745.481 216.466 402.475 163.982 585 125 275 91
13 -1.27E+00 0 1100.15 187.909 1910.6 188.975 902 106 1548 100
14 6.64E-02 0 197.048 178.062 178.729 172.459 111 92.5 96 92
15 -8.34E-03 0 250.674 207.943 210.682 170.287 137 118 110 102
16 1.22E-02 0 211.526 197.094 193.94 193.047 116 95 98.5 98.5
17 -1.28E-01 0 431.887 191.473 432.594 172.126 307 102.5 268 91.5
18 -4.36E-01 0 929.437 203.752 1078.91 203.207 782 101 852 104
19 -3.70E-01 0 184.703 190.417 187.991 189.792 93 107 101 97
20 -6.64E-02 0 313.558 187.282 284.768 129.134 181 95.5 151 86

Total number of rows: 16928

Table truncated, full table size 1089 Kbytes.




Supplementary data files not provided

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