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Sample GSM22727 Query DataSets for GSM22727
Status Public on May 21, 2004
Title A chip: time 3 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.65E-01 0 97.2956 65.7117 95.7774 78.6014 22 12 27 13
2 -8.73E-02 0 73.9504 59.1932 80.1674 79.8598 10 10 17 12
3 7.00E-01 0 224.206 79.3083 216.558 95.1053 130.5 17 143 25
4 -2.74E-01 0 84.4161 67.473 108.571 71.3716 18 11 35.5 12
5 -3.15E-01 0 739.172 109.937 1390.12 110.146 617 29 1042 37.5
6 2.51E-01 0 95.3096 68.124 90.9263 72.756 19 10 18 12.5
7 -3.49E-01 0 83.9209 57.7261 117.737 66.7357 15 8 32 11
8 3.39E-01 0 94.4244 78.4211 76.6395 70.2256 23 15 10 12
9 3.45E-01 0 566.385 91.8782 712.119 85.2941 404 18.5 431 17.5
10 -2.63E-02 0 78.9532 73.7184 90.6503 73.5345 17 11 25 20.5
11 -2.72E-01 0 121.184 56.1939 204.146 88.6224 28 10 92 25.5
12 NULL 0 77.2664 62.7833 128.214 64.3498 12 10 39 10
13 2.18E-01 0 118.664 65.5486 118.942 57 26 11 36 9
14 1.76E-02 0 74.722 73.5414 70.728 60.8232 10 13 9 10
15 -1.30E-01 0 73.9186 61.797 85.3576 73.1203 11 10 15.5 15
16 -2.39E-01 0 72.1547 67.3522 85.5612 69.8866 10 9 20.5 10
17 -6.80E-02 0 146.624 54.9439 218.071 89.2706 51 10 111 15
18 -2.45E-02 0 253.842 80.125 393.698 69.4964 148 17 282 8
19 -2.46E-02 0 76.3562 46.9853 81.0923 53.9228 14 7 15 9
20 NULL 0 63.7302 54.1567 95.1297 83.7788 9 7 27 13

Total number of rows: 16928

Table truncated, full table size 1032 Kbytes.




Supplementary data files not provided

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