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Sample GSM22730 Query DataSets for GSM22730
Status Public on May 21, 2004
Title A chip: time 3 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -1.37E-01 0 79.7938 59.6646 98.3869 70.8671 15 8 25.5 9
2 -4.64E-01 0 83.3596 64.9645 130.251 79.3652 20 10 43 14
3 4.18E-01 0 459.957 82.9444 497.457 97.4841 301 16.5 339 19
4 -6.26E-02 0 90.043 78.9752 116.166 85.3865 22.5 8.5 34.5 20
5 -5.86E-01 0 1602.17 122.208 3107.19 131.517 1447.5 33.5 2891.5 59.5
6 2.70E-01 0 121.262 61.8587 134.105 87.9591 35 9 40.5 17
7 -2.60E-01 0 92.8088 45.5777 133.532 66.9662 20 7 53 12
8 -3.89E-01 0 56.6207 64.0284 74.6355 59.4716 8 12.5 18.5 8.5
9 -2.69E-01 0 363.828 71.06 648.252 106.24 251.5 13.5 486.5 35.5
10 -1.12E-01 0 71.75 61.5987 88.7956 76.9547 12 8 17 12
11 -6.69E-01 0 96.5875 60.9659 192.455 50.572 25 9 93.5 8
12 6.98E-01 0 157.976 63.556 141.38 75.6791 49.5 8.5 42 20
13 -1.68E-01 0 190.288 60.097 307.122 66.5522 82.5 8 113.5 10.5
14 3.69E-01 0 88.9185 73.9776 80.5 64.0522 21.5 10 17 11.5
15 4.79E-01 0 85.731 51.771 75.837 74.743 18 6 17.5 12
16 4.18E-02 0 83.7706 57.32 99.1912 94.0222 15 7 20.5 15
17 1.20E-01 0 211.44 77.7077 275.656 76.6056 121.5 15 170.5 16
18 -1.19E-01 0 334.823 90.695 521.635 107.266 229 20 399 18.5
19 -1.09E-02 0 70.9544 71.3734 75.8759 59.7152 11 9 16 8
20 6.01E-01 0 91.9853 65.1894 78.2824 86.8598 22.5 10 14 18.5

Total number of rows: 16928

Table truncated, full table size 1045 Kbytes.




Supplementary data files not provided

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