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Sample GSM22732 Query DataSets for GSM22732
Status Public on May 21, 2004
Title A chip: time 3 hour Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -2.80E-01 0 77.4849 61.7059 122.984 81.0727 12 9 43 20
2 -2.58E-01 0 85.1479 59.5991 139.065 103.694 16 7 46 23
3 -2.17E-01 0 255.618 56.1004 384.81 87.0743 151 8 308 25
4 -5.08E-01 0 75.502 68.0355 140.222 99.7234 12.5 9 67 23
5 2.53E-02 0 1258.75 100.671 1504.01 131.168 1072 28 1312 56
6 1.58E-01 0 141.087 65.3403 177.076 81.9757 47.5 8 87 17
7 -5.24E-01 0 88.765 69.9547 150.377 63.4302 19 9 59 8
8 3.31E-01 0 64.051 65.75 76.8506 88.5379 7 9 12 18.5
9 -2.29E-01 0 654.163 77.0164 972.244 116.461 482 13.5 754 33
10 -2.45E-01 0 91.9873 45.3669 143.959 82.7266 10 7 41 19
11 -9.14E-01 0 142.393 50.4539 334.371 90.2796 58.5 7 227 15
12 -6.23E-01 0 62.2397 52.7703 116.89 80.598 8 7 35 12.5
13 -2.60E-01 0 124.916 53.7621 199.368 72.869 39 7 74.5 12
14 NULL 0 57.1392 41.5576 112.352 66.8756 7 6 44 9
15 -2.55E-01 0 57.5291 46.7361 95.4506 82.4444 7 5 18 15
16 NULL 0 72.7409 55.85 115.606 79.7233 8 6.5 31 19
17 -5.69E-01 0 114.334 50.9369 217.652 59.0694 27 7 121 8
18 -4.66E-01 0 302.583 58.5347 526.959 68.1944 220 7 428.5 10
19 -1.54E-01 0 66.638 49.6723 97.6152 84.034 8 7 26 14
20 5.94E-01 0 70.0707 44.8357 86.5289 98.7286 10 5 18 29.5

Total number of rows: 16928

Table truncated, full table size 1026 Kbytes.




Supplementary data files not provided

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