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Sample GSM22733 Query DataSets for GSM22733
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -4.22E-01 0 94.3196 59.8157 113.765 59.898 19 8 36 8
2 4.49E-01 0 96.7088 69.0133 61.606 47.9967 27 12 11 7
3 5.24E-01 0 391.557 57.6511 305.202 77.3525 287 7.5 199 17.5
4 -3.16E-01 0 88.372 79.3185 97.1017 86.5185 17 13.5 20 13
5 6.37E-01 0 1137.81 108.316 687.636 90.398 958 25.5 592 28
6 NULL 0 98.2262 75.1404 127.188 79.0308 22.5 13.5 47 14.5
7 -6.53E-01 0 88.0482 80.5764 108.606 64.2083 19 17 29.5 9
8 1.82E-01 0 87.8761 49.4909 71.1147 58.6636 14 7.5 9 8
9 -1.07E+00 0 476.306 82.0479 948.665 80.3801 358.5 15.5 678 17
10 NULL 0 87.7798 69.3421 91.6687 71.2434 14 11 20.5 11
11 -7.06E-01 0 129.878 78.0809 203.811 81.9515 39 10 92 17
12 5.48E-01 0 175.795 82.2103 113.849 59.1111 54 15 28 10
13 -4.85E-01 0 145.73 64.5434 189.454 64.1918 58 12 70 10
14 1.73E-01 0 71.3234 79.5514 74.7092 72.4074 14 10 9 20
15 2.32E-02 0 87.8424 51.354 78.212 53.1204 19 8 9.5 7
16 2.10E-01 0 96.0593 62.4021 81.6884 73.1376 18 11 12 15
17 1.62E-01 0 200.144 69.3309 180.03 76.4388 108 8.5 96 14
18 -9.85E-02 0 266.397 64.2093 294.839 75.4535 151 12 188.5 10
19 4.02E-01 0 84.4176 56.7831 66.7002 72.7757 11 10 11 15
20 -2.14E-01 0 97.4817 63.1076 100.748 64.9792 23.5 8 30 7

Total number of rows: 16928

Table truncated, full table size 1023 Kbytes.




Supplementary data files not provided

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