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Sample GSM22734 Query DataSets for GSM22734
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -4.39E-01 0 97.806 67.054 118.677 66.7446 21 13 37 10
2 3.95E-01 0 112 65.5296 90.3095 76.0987 35.5 10 21 19
3 -1.43E-01 0 634.145 93.6071 839.356 100.27 514 27.5 656 29.5
4 -2.16E-01 0 114.95 79.3224 142.143 87.8191 35 10 50 19
5 7.01E-01 0 3501.92 129.566 2032.95 130.427 3225.5 36 1708.5 48
6 -1.16E-01 0 146.133 82.2857 162.693 72.1865 62 15 73 15
7 -1.01E-01 0 134.887 83.0897 143.803 67.2379 52.5 13 58 9
8 NULL 0 74.1799 63.971 77.9422 83.6377 13 10 17 14
9 -5.77E-01 0 867.099 168.809 1381.56 148.511 728 65 1110 59.5
10 2.44E-01 0 110.847 69.4783 94.7923 89.9348 35 12 20 18
11 -6.79E-01 0 228.69 83.7118 418.294 87.4653 134 15.5 356 18.5
12 NULL 0 131.614 76.7619 140.507 67.3373 31 13.5 45 11
13 -4.99E-01 0 160.157 75.3547 241.763 79.141 64 14.5 111 22.5
14 -3.53E-01 0 72.3024 58.1236 84.5574 71.5891 11 10 20 13
15 NULL 0 92.144 69.5388 105.541 65.6408 19.5 14.5 27 11
16 -1.18E-01 0 98.3294 88.7725 90.9911 69.3704 20 20 19 13
17 5.08E-02 0 337.856 80.125 393.929 72.5799 249.5 10.5 276.5 14
18 -5.61E-01 0 457.08 73.0683 832.291 75.3849 369.5 12.5 639 11
19 -3.91E-02 0 85.5974 61.2706 83.4845 75.7765 17 11 18 14
20 1.14E-02 0 89.496 77.6781 81.373 73.3185 17.5 12 18 11.5

Total number of rows: 16928

Table truncated, full table size 1035 Kbytes.




Supplementary data files not provided

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