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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
3.59E-01
0
223.816
185.526
354.049
307.855
129
74
280
243
2
1.09E+00
0
401.949
350.865
521.658
540.582
312
237.5
440.5
451.5
3
5.92E-01
0
2019.1
227.787
1305.95
251.123
1650
161
1068
172
4
3.92E-01
0
234.922
189.654
207.574
191.028
153
102
135
115
5
1.15E-01
0
2559.67
202.738
2128.5
205.338
2383
131
1772
141
6
3.51E-02
0
401.362
167.967
391.989
181.581
336
104
305
110.5
7
-5.14E-01
0
197.876
156.93
244.423
158.674
127
74
140
75.5
8
1.39E-01
0
173.052
150.523
162.645
167.073
94
63.5
78
71.5
9
-6.73E-01
0
993.796
153.496
1456.14
157.233
814
79.5
1112
65
10
-2.17E-01
0
179.565
144.135
192.579
153.964
91
67
121
71
11
-7.28E-01
0
267.364
172.644
343.568
177.261
178
103
252
83
12
4.03E-01
0
554.565
190.239
426.514
170.628
393
100
308
81
13
-5.14E-01
0
951.044
168.789
1326.07
197.235
723
84
1012
131
14
-5.61E-01
0
155.249
165.426
160.972
153.115
77
85.5
83
64.5
15
9.33E-03
0
181.002
159.3
189.414
189.58
113
84
113.5
100
16
4.72E-01
0
193.634
153.286
193.569
175.734
96
78
107
117
17
-4.50E-01
0
357.911
167.055
434.615
156.534
262.5
90
318
76
18
-2.67E-01
0
761.55
155.453
911.583
177.255
621
77.5
750
86
19
1.86E-01
0
158.688
187.701
167.488
179.022
77
88
88
110
20
-2.50E-01
0
241.992
160.302
246.584
159.247
149
86
170.5
69.5
Total number of rows: 16928 Table truncated, full table size 1069 Kbytes .Supplementary data files not provided