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Sample GSM22738 Query DataSets for GSM22738
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 3.59E-01 0 223.816 185.526 354.049 307.855 129 74 280 243
2 1.09E+00 0 401.949 350.865 521.658 540.582 312 237.5 440.5 451.5
3 5.92E-01 0 2019.1 227.787 1305.95 251.123 1650 161 1068 172
4 3.92E-01 0 234.922 189.654 207.574 191.028 153 102 135 115
5 1.15E-01 0 2559.67 202.738 2128.5 205.338 2383 131 1772 141
6 3.51E-02 0 401.362 167.967 391.989 181.581 336 104 305 110.5
7 -5.14E-01 0 197.876 156.93 244.423 158.674 127 74 140 75.5
8 1.39E-01 0 173.052 150.523 162.645 167.073 94 63.5 78 71.5
9 -6.73E-01 0 993.796 153.496 1456.14 157.233 814 79.5 1112 65
10 -2.17E-01 0 179.565 144.135 192.579 153.964 91 67 121 71
11 -7.28E-01 0 267.364 172.644 343.568 177.261 178 103 252 83
12 4.03E-01 0 554.565 190.239 426.514 170.628 393 100 308 81
13 -5.14E-01 0 951.044 168.789 1326.07 197.235 723 84 1012 131
14 -5.61E-01 0 155.249 165.426 160.972 153.115 77 85.5 83 64.5
15 9.33E-03 0 181.002 159.3 189.414 189.58 113 84 113.5 100
16 4.72E-01 0 193.634 153.286 193.569 175.734 96 78 107 117
17 -4.50E-01 0 357.911 167.055 434.615 156.534 262.5 90 318 76
18 -2.67E-01 0 761.55 155.453 911.583 177.255 621 77.5 750 86
19 1.86E-01 0 158.688 187.701 167.488 179.022 77 88 88 110
20 -2.50E-01 0 241.992 160.302 246.584 159.247 149 86 170.5 69.5

Total number of rows: 16928

Table truncated, full table size 1069 Kbytes.




Supplementary data files not provided

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