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Sample GSM22740 Query DataSets for GSM22740
Status Public on May 21, 2004
Title A chip: time 24 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -2.32E-01 0 276.694 248.434 248.062 231.155 206 158 171 126
2 NULL 0 493.762 409.361 479.437 463.258 447 330 414 342
3 7.18E-01 0 1590.74 240.087 723.765 292.408 1270 153 593 212
4 1.48E-01 0 199.269 162.063 207.908 208.211 137 85 131 117
5 -9.21E-02 0 1355.14 203.014 846.26 213.413 1163 130 723 135
6 -7.07E-02 0 269.166 179.757 238.131 172.686 185 94 168 99
7 -2.94E-01 0 228.843 187.511 194.497 146.932 145 91 102 59
8 4.74E-01 0 191.617 167.906 149.468 151.536 107 76 69 73
9 -1.69E-01 0 685.381 210.549 476.778 167.415 567 130 379 85
10 -8.25E-01 0 190.916 190.721 164.413 137.721 107 118.5 73 55
11 -6.50E-01 0 208.676 159.209 210.714 146.566 142 96.5 138 65
12 6.08E-01 0 689.04 167.261 362.75 152.417 507 92.5 247 96
13 -3.69E-01 0 633.721 185.3 456.413 131.319 506 127 364 49
14 -1.76E-01 0 185.375 172.149 156.413 152 115 94 75.5 65
15 -6.61E-01 0 188.118 207.456 154.284 159.592 105 135 78 78
16 -2.19E-01 0 186.601 220.323 159.553 162.177 103 116.5 79 87
17 -3.31E-01 0 269.073 184.708 200.847 112.461 173 74 112 22
18 -1.06E-01 0 433.339 178.869 298.805 142.59 353 114.5 221 55.5
19 2.24E-01 0 170.931 161.091 146.869 165.201 87 84 71 80
20 1.96E-01 0 224.216 174.086 171.012 153.4 159.5 87 99.5 63.5

Total number of rows: 16928

Table truncated, full table size 1086 Kbytes.




Supplementary data files not provided

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