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Sample GSM22746 Query DataSets for GSM22746
Status Public on May 21, 2004
Title A chip: time 3 day Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -0.166152545 0 574.301 403.238 380.53 313.494 471 279 232 166
2 -0.753212258 0 603.394 738.029 476.812 516.859 481.5 565 357 383
3 0.507358999 0 2777.28 455.96 1115.26 400.355 2179 335 885 292
4 -1.077896786 0 434.846 357.639 356.32 244.024 344 292 220 140
5 -1.203135748 0 5411.94 394.332 4437.89 314.742 5119 277 4012 205
6 -0.410125668 0 690.2 317.378 520.488 231.369 591 193 362 113
7 -0.705441599 0 502.671 332.876 387.343 225.324 380 234 254 109
8 -0.080305747 0 303.197 313.988 236.033 231.096 177.5 200.5 134 130
9 -1.474527923 0 2331.14 351.813 2559.28 239.683 2079 241.5 2258 149
10 -0.11356547 0 391.153 318.717 287.336 223.756 269 185.5 182 128
11 -0.390288993 0 653.619 361.048 496.696 237.102 530 220 342 144
12 0.899541146 0 1253.49 281.335 470.574 219.423 1034 172 334 123.5
13 -0.704221739 0 4115.31 324.013 2605.99 245.516 4097 202 2471 145
14 0.004033726 0 303.452 289.313 234.04 215.796 198 170 127 120.5
15 0.447239107 0 358.115 230.703 250.62 220.928 250 138 135 132
16 -0.375221505 0 394.293 290.301 320.776 249.558 266 182 198 130
17 -0.257634536 0 1076.25 326.979 728.981 222.878 918.5 202 537.5 122.5
18 -0.971998419 0 2259.85 326.301 1898.96 266.29 2098 209 1758 150
19 0.077038391 0 402.22 314.105 263.616 227.341 283 195.5 143 121.5
20 0.135460546 0 581.011 327.4 362.401 267.895 465 218 219 152

Total number of rows: 16928

Table truncated, full table size 1151 Kbytes.




Supplementary data files not provided

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