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Sample GSM22764 Query DataSets for GSM22764
Status Public on May 21, 2004
Title B chip Version1: time 30 min Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 187.612 193.112 131.207 132.286 109 133 48 39
2 4.44E-01 0 246.809 197.313 162.461 157.307 172 112 77 63
3 4.21E-02 0 1178.01 218.954 919.166 158.625 992 137 743 53
4 -4.08E-01 0 194.871 217.622 135.699 147.324 111 132 59 47
5 1.39E-01 0 812.087 224.227 583.228 137.152 691 141 445 69.5
6 -2.10E-01 0 372.161 204.396 340.003 131.165 297 112 250.5 53
7 1.68E-01 0 224.303 177.097 163.272 111.483 153.5 90 79.5 41.5
8 -3.25E-02 0 259.043 177.547 204.547 143.056 187 103 129 53
9 -1.29E-01 0 359.909 213.857 282.984 151.436 279 145 186 62
10 -5.73E-01 0 205.555 191.463 158.94 127.547 134.5 122 73.5 39
11 2.24E-01 0 253.989 192.925 165.612 121.644 183 104 84 37
12 -5.21E-02 0 217.014 192.419 156.384 117.662 151.5 107.5 65 45.5
13 4.11E-01 0 263.403 177.991 179.49 136.026 193 108 87 61
14 -7.69E-01 0 213.113 203.519 181.435 119.963 123 124.5 104 36.5
15 NULL 1 328.146 223.904 351.108 142.687 242 139.5 208 62.5
16 NULL 1 281.933 202.176 234.965 156.869 172 106 118 72
17 -1.16E-01 0 347.024 182.107 278.04 135.883 267 128 177 54
18 -8.81E-02 0 229.077 213.388 176.687 132.764 149 115.5 93.5 57.5
19 -7.26E-01 0 234.601 199.929 203.344 108.694 160 137 109 43.5
20 9.23E-01 0 209.435 172.282 158.109 162.376 124 98 75 96

Total number of rows: 14720

Table truncated, full table size 936 Kbytes.




Supplementary data files not provided

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