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Status
Public on May 21, 2004
Title
B chip Version1: time 30 min Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
187.612
193.112
131.207
132.286
109
133
48
39
2
4.44E-01
0
246.809
197.313
162.461
157.307
172
112
77
63
3
4.21E-02
0
1178.01
218.954
919.166
158.625
992
137
743
53
4
-4.08E-01
0
194.871
217.622
135.699
147.324
111
132
59
47
5
1.39E-01
0
812.087
224.227
583.228
137.152
691
141
445
69.5
6
-2.10E-01
0
372.161
204.396
340.003
131.165
297
112
250.5
53
7
1.68E-01
0
224.303
177.097
163.272
111.483
153.5
90
79.5
41.5
8
-3.25E-02
0
259.043
177.547
204.547
143.056
187
103
129
53
9
-1.29E-01
0
359.909
213.857
282.984
151.436
279
145
186
62
10
-5.73E-01
0
205.555
191.463
158.94
127.547
134.5
122
73.5
39
11
2.24E-01
0
253.989
192.925
165.612
121.644
183
104
84
37
12
-5.21E-02
0
217.014
192.419
156.384
117.662
151.5
107.5
65
45.5
13
4.11E-01
0
263.403
177.991
179.49
136.026
193
108
87
61
14
-7.69E-01
0
213.113
203.519
181.435
119.963
123
124.5
104
36.5
15
NULL
1
328.146
223.904
351.108
142.687
242
139.5
208
62.5
16
NULL
1
281.933
202.176
234.965
156.869
172
106
118
72
17
-1.16E-01
0
347.024
182.107
278.04
135.883
267
128
177
54
18
-8.81E-02
0
229.077
213.388
176.687
132.764
149
115.5
93.5
57.5
19
-7.26E-01
0
234.601
199.929
203.344
108.694
160
137
109
43.5
20
9.23E-01
0
209.435
172.282
158.109
162.376
124
98
75
96
Total number of rows: 14720 Table truncated, full table size 936 Kbytes .Supplementary data files not provided