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Status
Public on May 21, 2004
Title
B chip Version1: time 30 min Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
273.215
260.057
264.389
300.348
162.5
132
161
164
2
-3.54E-01
0
318.632
318.341
309.59
245.618
197
200
191
147
3
1.79E-02
0
844.734
332.601
896.908
273.072
676.5
218
674.5
165
4
-2.83E-01
0
281.865
291.461
285.59
282.122
167
184.5
173.5
162.5
5
1.75E-01
0
595.078
309.196
667.839
375.042
446.5
182.5
496
234
6
-6.00E-02
0
361.598
317.753
391.523
279.359
240
185
249
173
7
-5.05E-01
0
297.019
333.41
302.211
285.174
184
210
175
173.5
8
-3.48E-01
0
295.81
278.659
337.504
254.09
182
159
235
147
9
-5.31E-02
0
344.82
264.642
377.435
276.387
218
170
245
163
10
-3.01E-01
0
292.108
277.929
325.921
246.182
182
164.5
195
156
11
8.92E-01
0
268.846
264.944
241.828
280.684
143.5
138
127.5
174
12
-3.38E-01
0
230.238
264.974
257.356
279.487
120
146
155.5
153.5
13
3.21E-01
0
262.255
225.67
285.996
297.085
150.5
118.5
173.5
169
14
-4.45E-01
0
243.381
233.129
290.818
249.641
122.5
129
186
124
15
-9.59E-01
0
277.229
279.061
374.049
345.813
149
198.5
229
214
16
-1.25E-01
0
236.948
246.81
299.793
326.661
133
147.5
182
205.5
17
-1.39E-02
0
327.898
238.132
411.9
301.83
211
131
254
172
18
-6.11E-01
0
224.518
232.678
246.395
238.722
127
146
135
127
19
-5.73E-01
0
224.245
246.5
279.775
266.936
127
143
174
157.5
20
-6.50E-02
0
255.588
277.182
303.632
311.561
148
145
192
185
Total number of rows: 14720 Table truncated, full table size 953 Kbytes .Supplementary data files not provided