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Sample GSM22765 Query DataSets for GSM22765
Status Public on May 21, 2004
Title B chip Version1: time 30 min Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 273.215 260.057 264.389 300.348 162.5 132 161 164
2 -3.54E-01 0 318.632 318.341 309.59 245.618 197 200 191 147
3 1.79E-02 0 844.734 332.601 896.908 273.072 676.5 218 674.5 165
4 -2.83E-01 0 281.865 291.461 285.59 282.122 167 184.5 173.5 162.5
5 1.75E-01 0 595.078 309.196 667.839 375.042 446.5 182.5 496 234
6 -6.00E-02 0 361.598 317.753 391.523 279.359 240 185 249 173
7 -5.05E-01 0 297.019 333.41 302.211 285.174 184 210 175 173.5
8 -3.48E-01 0 295.81 278.659 337.504 254.09 182 159 235 147
9 -5.31E-02 0 344.82 264.642 377.435 276.387 218 170 245 163
10 -3.01E-01 0 292.108 277.929 325.921 246.182 182 164.5 195 156
11 8.92E-01 0 268.846 264.944 241.828 280.684 143.5 138 127.5 174
12 -3.38E-01 0 230.238 264.974 257.356 279.487 120 146 155.5 153.5
13 3.21E-01 0 262.255 225.67 285.996 297.085 150.5 118.5 173.5 169
14 -4.45E-01 0 243.381 233.129 290.818 249.641 122.5 129 186 124
15 -9.59E-01 0 277.229 279.061 374.049 345.813 149 198.5 229 214
16 -1.25E-01 0 236.948 246.81 299.793 326.661 133 147.5 182 205.5
17 -1.39E-02 0 327.898 238.132 411.9 301.83 211 131 254 172
18 -6.11E-01 0 224.518 232.678 246.395 238.722 127 146 135 127
19 -5.73E-01 0 224.245 246.5 279.775 266.936 127 143 174 157.5
20 -6.50E-02 0 255.588 277.182 303.632 311.561 148 145 192 185

Total number of rows: 14720

Table truncated, full table size 953 Kbytes.




Supplementary data files not provided

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