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Status
Public on May 21, 2004
Title
B chip Version1: time 30 min Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
194.671
164.32
193.423
171.864
106.5
88.5
126.5
83
2
NULL
0
207.339
201.782
259.99
198.691
128
149
220
130
3
4.69E-02
0
1018.79
183.719
1053.56
237.044
883
121
893
167
4
-1.47E-01
0
198.36
189.418
197.658
158.718
121
107.5
116
94
5
3.19E-01
0
722.845
177.516
633.831
151.866
614
82
527
103
6
1.91E-01
0
373.615
170.567
358.38
176.062
287
115
273
109
7
1.55E-01
0
212.432
173.564
208.874
190.153
134
119
152
123.5
8
-1.62E-01
0
242.854
212.545
262.622
224.026
171
146
174
151
9
-1.32E-01
0
313
193.763
340.581
188.778
226
117.5
248
116.5
10
NULL
0
193.202
159.578
241.889
205.516
108
73
180
136.5
11
6.70E-02
0
283.107
169.56
260.355
178.669
192
115.5
170
101.5
12
1.97E-01
0
216.242
188.37
219.399
180.782
143
112
145
126
13
4.54E-01
0
221.752
159.43
243.173
225.164
148
86.5
163
142.5
14
-4.62E-01
0
189.122
187.846
224.774
186.413
99
112.5
153
116.5
15
1.25E-01
0
301.481
179.684
319.7
177.947
241.5
77.5
211
103
16
-4.79E-01
0
212.565
171.958
264.475
183.446
123
93
183
97
17
-1.59E-01
0
336.444
194.104
390.2
192.533
255
122.5
291
132.5
18
-7.11E-01
0
184.224
198.519
226.273
222.638
113
127
146
129
19
-3.15E-01
0
230.383
201.067
247.607
196.417
170
134
162
124
20
2.26E-01
0
218.938
203.044
243.494
229.764
142
109
175
147
Total number of rows: 14720 Table truncated, full table size 947 Kbytes .Supplementary data files not provided