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Sample GSM22767 Query DataSets for GSM22767
Status Public on May 21, 2004
Title B chip Version1: time 30 min Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 194.671 164.32 193.423 171.864 106.5 88.5 126.5 83
2 NULL 0 207.339 201.782 259.99 198.691 128 149 220 130
3 4.69E-02 0 1018.79 183.719 1053.56 237.044 883 121 893 167
4 -1.47E-01 0 198.36 189.418 197.658 158.718 121 107.5 116 94
5 3.19E-01 0 722.845 177.516 633.831 151.866 614 82 527 103
6 1.91E-01 0 373.615 170.567 358.38 176.062 287 115 273 109
7 1.55E-01 0 212.432 173.564 208.874 190.153 134 119 152 123.5
8 -1.62E-01 0 242.854 212.545 262.622 224.026 171 146 174 151
9 -1.32E-01 0 313 193.763 340.581 188.778 226 117.5 248 116.5
10 NULL 0 193.202 159.578 241.889 205.516 108 73 180 136.5
11 6.70E-02 0 283.107 169.56 260.355 178.669 192 115.5 170 101.5
12 1.97E-01 0 216.242 188.37 219.399 180.782 143 112 145 126
13 4.54E-01 0 221.752 159.43 243.173 225.164 148 86.5 163 142.5
14 -4.62E-01 0 189.122 187.846 224.774 186.413 99 112.5 153 116.5
15 1.25E-01 0 301.481 179.684 319.7 177.947 241.5 77.5 211 103
16 -4.79E-01 0 212.565 171.958 264.475 183.446 123 93 183 97
17 -1.59E-01 0 336.444 194.104 390.2 192.533 255 122.5 291 132.5
18 -7.11E-01 0 184.224 198.519 226.273 222.638 113 127 146 129
19 -3.15E-01 0 230.383 201.067 247.607 196.417 170 134 162 124
20 2.26E-01 0 218.938 203.044 243.494 229.764 142 109 175 147

Total number of rows: 14720

Table truncated, full table size 947 Kbytes.




Supplementary data files not provided

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