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Sample GSM22768 Query DataSets for GSM22768
Status Public on May 21, 2004
Title B chip Version1: time 30 min Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 203.127 244.989 226.113 201.912 124.5 183 157 134.5
2 -9.48E-02 0 251.328 228.1 223.39 190.965 152 162 159 119
3 1.89E-01 0 1166.1 240.027 1132.41 243.625 988 154 899 159
4 -1.46E+00 0 241.601 257.706 229.572 212.254 153 210 157 118
5 8.79E-02 0 814.658 241.793 795.12 211.917 716 159 662 138
6 1.92E-01 0 488.25 234.933 495.087 227.384 386 159 385 165
7 1.11E+00 0 287.701 228.162 223.825 219.347 198 166.5 155 160.5
8 2.54E-01 0 307.963 248.414 277.79 221.949 221 164 211 149.5
9 3.53E-02 0 399.583 247.295 392.461 224.132 305 175 295 147
10 -3.96E-01 0 263.644 251.563 249.418 204.904 198 173 183 124
11 1.06E-01 0 268.428 235.484 260.219 221.214 190.5 150.5 181.5 147.5
12 2.74E-01 0 270.051 244.77 234.3 227.145 211 180 160 150
13 8.00E-01 0 295.927 259.18 238.381 222.776 206 185 165 167
14 2.59E-01 0 261.874 217.396 230.587 199.608 185 144 154 128.5
15 1.84E-01 0 402.984 221.731 377.862 209.908 326 150 252 126
16 -9.10E-01 0 258.144 252.753 279.785 206.62 178 193.5 199 145
17 1.38E-01 0 423.344 230.028 386.182 182.381 337 163 312 117.5
18 -3.95E-01 0 250.974 232.971 224.638 195.463 204 160 146 99
19 6.51E-01 0 267.066 208.656 228.836 198.248 181 119 152 132
20 -2.04E-01 0 249.495 239.99 242.45 202.337 186 170 159 140

Total number of rows: 14720

Table truncated, full table size 947 Kbytes.




Supplementary data files not provided

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