NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22769 Query DataSets for GSM22769
Status Public on May 21, 2004
Title B chip Version1: time 1 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 160.109 138.495 174.852 162.808 77 56.5 106 93.5
2 NULL 0 165.867 165.359 189.92 168.878 85 87 123 112
3 4.64E-01 0 1795.07 194.563 1246.47 206.322 1206 112 925 121.5
4 -4.89E-01 0 205.673 190.904 214.493 174.442 110.5 121 143 98.5
5 2.03E-01 0 1200.32 187.479 1047.37 197.398 1093 108 871 107.5
6 -2.92E-01 0 416.224 154.242 551.077 159.648 333 90 441 97.5
7 -1.69E-01 0 240.738 160.153 258.985 153.277 141 95 189 85
8 1.27E-01 0 217.875 133.893 267.837 196.068 148.5 74.5 162.5 129.5
9 8.38E-02 0 320.969 140.61 386.63 198.655 220.5 57.5 266.5 118
10 4.93E-02 0 208 154.489 214.989 158.258 132 73.5 130 79
11 NULL 0 197.144 143.611 230.933 168.057 117 71 143 88
12 -1.13E-01 0 180.439 164.963 212.715 188.537 98 84.5 137 111.5
13 5.46E-02 0 214.284 147.226 222.177 147.644 132 75.5 139 81.5
14 -1.76E-01 0 165.653 148.157 195.374 174.245 84 78 127 98
15 -9.00E-01 0 280.506 158.862 475.614 167.348 187 84.5 391 76
16 -3.92E-01 0 210.211 167.918 279.293 178.758 131 80.5 205 98.5
17 -5.50E-01 0 318.727 179.215 439.514 188.319 243 109 353 108
18 -4.71E-01 0 207.882 180.382 250.714 161.879 115 91.5 191 80.5
19 -2.91E-01 0 193.395 202.438 196.69 193.022 102 139 131 129
20 2.59E-01 0 177.625 151.656 204.065 164.758 99.5 61 135 109

Total number of rows: 14720

Table truncated, full table size 943 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap