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Status
Public on May 21, 2004
Title
B chip Version1: time 1 hour Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
160.109
138.495
174.852
162.808
77
56.5
106
93.5
2
NULL
0
165.867
165.359
189.92
168.878
85
87
123
112
3
4.64E-01
0
1795.07
194.563
1246.47
206.322
1206
112
925
121.5
4
-4.89E-01
0
205.673
190.904
214.493
174.442
110.5
121
143
98.5
5
2.03E-01
0
1200.32
187.479
1047.37
197.398
1093
108
871
107.5
6
-2.92E-01
0
416.224
154.242
551.077
159.648
333
90
441
97.5
7
-1.69E-01
0
240.738
160.153
258.985
153.277
141
95
189
85
8
1.27E-01
0
217.875
133.893
267.837
196.068
148.5
74.5
162.5
129.5
9
8.38E-02
0
320.969
140.61
386.63
198.655
220.5
57.5
266.5
118
10
4.93E-02
0
208
154.489
214.989
158.258
132
73.5
130
79
11
NULL
0
197.144
143.611
230.933
168.057
117
71
143
88
12
-1.13E-01
0
180.439
164.963
212.715
188.537
98
84.5
137
111.5
13
5.46E-02
0
214.284
147.226
222.177
147.644
132
75.5
139
81.5
14
-1.76E-01
0
165.653
148.157
195.374
174.245
84
78
127
98
15
-9.00E-01
0
280.506
158.862
475.614
167.348
187
84.5
391
76
16
-3.92E-01
0
210.211
167.918
279.293
178.758
131
80.5
205
98.5
17
-5.50E-01
0
318.727
179.215
439.514
188.319
243
109
353
108
18
-4.71E-01
0
207.882
180.382
250.714
161.879
115
91.5
191
80.5
19
-2.91E-01
0
193.395
202.438
196.69
193.022
102
139
131
129
20
2.59E-01
0
177.625
151.656
204.065
164.758
99.5
61
135
109
Total number of rows: 14720 Table truncated, full table size 943 Kbytes .Supplementary data files not provided