NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22771 Query DataSets for GSM22771
Status Public on May 21, 2004
Title B chip Version1: time 1 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 216.033 209.783 270.319 288.882 118 120 169 169.5
2 -8.16E-01 0 199.976 237.654 293.816 264.259 115 127 190 154
3 7.67E-01 0 848.652 233.825 766.158 274.536 720 120 593 162
4 1.59E-02 0 229.073 228.232 272.305 249.389 120 109 159 135
5 1.00E-01 0 790.998 217.836 999.893 247.192 661 118 751 156
6 -4.61E-01 0 266.74 210.931 398.714 257.867 156 116 279 149
7 -4.60E-01 0 236.184 217.879 308.659 218.466 121 113.5 193 115
8 -2.91E-01 0 222.545 178.593 316.148 239.744 119 101 197 144
9 -4.45E-02 0 239.033 220.843 332.523 282.666 138 112 214 179
10 -8.46E-02 0 204.41 184.8 278.006 248.078 105 91 158 140
11 -2.79E-01 0 211.25 188.299 283.628 239.722 112 99.5 166 129
12 -3.01E-01 0 190.499 190.451 261.811 249.607 100 100.5 160 135.5
13 -6.44E-01 0 221.126 203.778 327.097 253.649 112 105 214 121
14 4.36E-02 0 218.659 218.021 296.046 264.945 119 109 177 172
15 -2.02E-01 0 241.666 188.863 354.375 285.55 129 106 221 173
16 -4.43E-01 0 226.978 216.037 324.029 260.845 127 115 211 149.5
17 -4.56E-01 0 263.332 207.68 373.639 266.047 157 121 247.5 144.5
18 -3.97E-01 0 199.564 176.07 307.704 255.939 112 93 180 149
19 NULL 0 204.334 215 286.609 314.249 103 119 176 235
20 -5.89E-01 0 194.611 206.125 280.962 264.412 108 113 175 143.5

Total number of rows: 14720

Table truncated, full table size 950 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap