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Status
Public on May 21, 2004
Title
B chip Version1: time 1 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
216.033
209.783
270.319
288.882
118
120
169
169.5
2
-8.16E-01
0
199.976
237.654
293.816
264.259
115
127
190
154
3
7.67E-01
0
848.652
233.825
766.158
274.536
720
120
593
162
4
1.59E-02
0
229.073
228.232
272.305
249.389
120
109
159
135
5
1.00E-01
0
790.998
217.836
999.893
247.192
661
118
751
156
6
-4.61E-01
0
266.74
210.931
398.714
257.867
156
116
279
149
7
-4.60E-01
0
236.184
217.879
308.659
218.466
121
113.5
193
115
8
-2.91E-01
0
222.545
178.593
316.148
239.744
119
101
197
144
9
-4.45E-02
0
239.033
220.843
332.523
282.666
138
112
214
179
10
-8.46E-02
0
204.41
184.8
278.006
248.078
105
91
158
140
11
-2.79E-01
0
211.25
188.299
283.628
239.722
112
99.5
166
129
12
-3.01E-01
0
190.499
190.451
261.811
249.607
100
100.5
160
135.5
13
-6.44E-01
0
221.126
203.778
327.097
253.649
112
105
214
121
14
4.36E-02
0
218.659
218.021
296.046
264.945
119
109
177
172
15
-2.02E-01
0
241.666
188.863
354.375
285.55
129
106
221
173
16
-4.43E-01
0
226.978
216.037
324.029
260.845
127
115
211
149.5
17
-4.56E-01
0
263.332
207.68
373.639
266.047
157
121
247.5
144.5
18
-3.97E-01
0
199.564
176.07
307.704
255.939
112
93
180
149
19
NULL
0
204.334
215
286.609
314.249
103
119
176
235
20
-5.89E-01
0
194.611
206.125
280.962
264.412
108
113
175
143.5
Total number of rows: 14720 Table truncated, full table size 950 Kbytes .Supplementary data files not provided