NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22772 Query DataSets for GSM22772
Status Public on May 21, 2004
Title B chip Version1: time 1 hour Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 235.889 293.956 231.457 228.335 124 181.5 123 120.5
2 -3.78E-01 0 269.553 282.814 264.862 257.535 146 173 140 138
3 7.82E-01 0 1425.95 279.605 872.729 250.177 1144 168 670 148
4 9.93E-02 0 257.725 241.786 236.316 219.399 149 143.5 126 127
5 5.66E-01 0 1168.93 283.331 806.004 222.706 1038 151.5 687 112
6 4.27E-01 0 477.395 253.063 415.069 272.241 331 146 299 154
7 1.01E-01 0 305.007 240.556 282.993 215.908 185 126 170 116
8 1.65E-01 0 301.851 223.176 291.183 233.257 174 118.5 165 127
9 1.21E-01 0 382.821 294.934 332.8 261.073 264 178 204 138
10 5.14E-01 0 265.024 217.646 234.69 245.726 143 125.5 132 133
11 3.09E-01 0 234.014 201.805 250.61 249.738 139 108 148 145.5
12 -7.88E-01 0 237.671 268.73 242.668 262.311 136 172.5 134 138.5
13 -1.07E-03 0 269.417 222.41 280.901 231.125 160 114 155 127
14 -3.80E-01 0 195.577 192.93 254.556 250.489 105 103 147 133
15 -4.41E-01 0 338.37 231.086 453.355 278.546 235 139 310 176
16 -4.45E-01 0 290.604 256.259 347.072 272.921 166 151 224 162.5
17 -7.05E-01 0 349.525 278.689 507.297 294.687 228 157 361 188
18 -3.92E-01 0 254.933 259.897 298.39 270.888 155 149.5 179 158
19 4.81E-01 0 273.188 223.985 266.538 255.757 162 112.5 156 147.5
20 -9.69E-02 0 266.002 235.52 273.233 227.076 157 130 146 126

Total number of rows: 14720

Table truncated, full table size 950 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap