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Sample GSM22776 Query DataSets for GSM22776
Status Public on May 21, 2004
Title B chip Version1: time 8 hour Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 121.166 110.043 149.313 142.516 43 29 70 78.5
2 2.62E-01 0 161.311 136.615 199.211 192.362 80 60 111 115
3 1.73E-01 0 641.548 129.147 605.918 172.231 540 70 487 93
4 NULL 0 160.843 127.097 207.749 169.251 88 48 114 94
5 -1.38E-01 0 1142.58 141.065 1164.56 200.091 1002 43 988 125
6 NULL 0 337.727 136.475 396.361 153.742 238 51.5 305 63.5
7 3.02E-01 0 249.494 107.253 274.955 187.329 168 44 166 110.5
8 NULL 0 218.796 141.946 255.926 171.5 110 72.5 195 107.5
9 4.32E-01 0 244.493 115.296 303.814 206.897 160 28 223 145
10 1.26E-02 0 140.723 113.988 188.847 152.373 60 34 108 86
11 4.64E-01 0 152.378 117.349 191.351 206.251 71 54 120 122
12 -8.39E-03 0 132.979 115.595 181.436 167.325 38 43 88 92
13 1.01E+00 0 258.562 119.812 204.021 180.495 178.5 39 123.5 97.5
14 -1.57E-01 0 152.985 140.435 185.707 154.738 75 69.5 114 94.5
15 5.70E-02 0 325.69 136 341.968 177.703 218 60 231 93
16 -5.42E-01 0 195.831 162.032 256.25 173.353 112 74 172 91
17 -4.38E-02 0 281.046 148.32 344.948 196.655 194 71 273 131.5
18 NULL 0 132.477 126.862 202.071 185.331 49 60 115 120
19 NULL 0 157.033 86.332 194.491 190.812 71.5 21 130.5 102
20 -8.97E-02 0 161.964 144.809 235.536 189.047 72 41 143 114.5

Total number of rows: 14720

Table truncated, full table size 933 Kbytes.




Supplementary data files not provided

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