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Status
Public on May 21, 2004
Title
B chip Version1: time 8 hour Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
121.166
110.043
149.313
142.516
43
29
70
78.5
2
2.62E-01
0
161.311
136.615
199.211
192.362
80
60
111
115
3
1.73E-01
0
641.548
129.147
605.918
172.231
540
70
487
93
4
NULL
0
160.843
127.097
207.749
169.251
88
48
114
94
5
-1.38E-01
0
1142.58
141.065
1164.56
200.091
1002
43
988
125
6
NULL
0
337.727
136.475
396.361
153.742
238
51.5
305
63.5
7
3.02E-01
0
249.494
107.253
274.955
187.329
168
44
166
110.5
8
NULL
0
218.796
141.946
255.926
171.5
110
72.5
195
107.5
9
4.32E-01
0
244.493
115.296
303.814
206.897
160
28
223
145
10
1.26E-02
0
140.723
113.988
188.847
152.373
60
34
108
86
11
4.64E-01
0
152.378
117.349
191.351
206.251
71
54
120
122
12
-8.39E-03
0
132.979
115.595
181.436
167.325
38
43
88
92
13
1.01E+00
0
258.562
119.812
204.021
180.495
178.5
39
123.5
97.5
14
-1.57E-01
0
152.985
140.435
185.707
154.738
75
69.5
114
94.5
15
5.70E-02
0
325.69
136
341.968
177.703
218
60
231
93
16
-5.42E-01
0
195.831
162.032
256.25
173.353
112
74
172
91
17
-4.38E-02
0
281.046
148.32
344.948
196.655
194
71
273
131.5
18
NULL
0
132.477
126.862
202.071
185.331
49
60
115
120
19
NULL
0
157.033
86.332
194.491
190.812
71.5
21
130.5
102
20
-8.97E-02
0
161.964
144.809
235.536
189.047
72
41
143
114.5
Total number of rows: 14720 Table truncated, full table size 933 Kbytes .Supplementary data files not provided