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Sample GSM22778 Query DataSets for GSM22778
Status Public on May 21, 2004
Title B chip Version1: time 24 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 221.241 170.277 196.014 184.325 118 91.5 103.5 103.5
2 -1.98E-01 0 254.468 250.561 261.842 227.358 166 144 159 142
3 -5.36E-02 0 1198.24 245.441 1014.27 225.226 1038 126 801.5 130
4 3.17E-01 0 255.754 207.329 244.569 222.106 144 105 138 133
5 4.25E-01 0 2214.4 263.816 1225.24 223.984 1970 140 1033 112
6 2.63E-01 0 559.002 229.608 418.071 197.379 460 117 272 110
7 -4.63E-02 0 433.683 249.7 360.809 189.359 290 143.5 232 101
8 -3.00E-01 0 258.856 198.186 283.958 208.199 137 105 155 114
9 1.14E-02 0 362.604 234.452 330.919 210.528 255 126 209 124
10 6.09E-02 0 229.278 181.875 223.068 213.181 118 96 117 104.5
11 -1.82E-02 0 216.28 209.981 223.547 205.757 111 111 121 120
12 -3.76E-01 0 228.809 217.934 233.616 199.668 113 124.5 122 105.5
13 1.53E-01 0 284.733 189.028 241.526 185.426 170 104.5 126 96.5
14 5.26E-02 0 223.979 212.748 215.123 223.761 126 110 115 110
15 -4.74E-01 0 378.601 208.07 426.917 202.43 244 104 269 101
16 -5.34E-02 0 303.486 256.747 309.115 278.811 193 152 177 166
17 9.40E-02 0 541.583 231.305 446.652 220.727 426 123 295 118
18 2.70E-01 0 293.464 233.254 228.908 215.546 169 130.5 123.5 105.5
19 1.08E-01 0 273.698 214.738 242.636 215.748 149 122.5 134 114
20 2.64E-01 0 243.244 252.042 214.179 252.521 143 155 112 133

Total number of rows: 14720

Table truncated, full table size 949 Kbytes.




Supplementary data files not provided

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