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Sample GSM22843 Query DataSets for GSM22843
Status Public on May 21, 2004
Title B chip Version2: time 3 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 152.122 86.125 174.748 131.984 59 28 74 63.5
2 NULL 0 183.974 153.117 170.048 162.168 114 70.5 85 87.5
3 2.39E+00 0 5217.65 249.65 1570.29 202.997 5099 183.5 1423 111.5
4 -2.33E-01 0 247.224 196.073 238.704 156.095 157.5 103 176.5 79
5 5.12E-01 0 1845.74 176.639 1726.9 190.715 1713 111 1622.5 89.5
6 1.16E-01 0 474.324 194.852 488.76 170.456 391 121 395 93
7 4.25E-01 0 6346.15 239.212 5819.43 259.667 6304 145 5834 182.5
8 2.58E-01 0 267.324 189.177 244.514 187.333 177 96.5 153 105
9 5.09E-01 0 373.139 135.332 355.165 183.661 298 66 264.5 104
10 -7.55E-02 0 186.938 162.235 160.809 125.714 112 75 69 53
11 3.46E-02 0 231.663 165 191.2 131.229 143 74.5 98 44
12 -2.11E-01 0 202.174 167.84 206.193 156.326 116.5 75.5 124.5 71
13 8.25E-01 0 650.923 162.56 507.666 166.883 572 66 429 90.5
14 -1.49E-01 0 214.427 171.563 216.718 160.87 115 87.5 150 86.5
15 4.56E-01 0 798.096 138.174 752.174 173.01 671 58 603 100
16 -1.38E+00 0 244.525 196.46 359.651 181.504 167 133.5 304.5 101.5
17 -4.44E-01 0 852.647 208.259 1262.27 214.827 773.5 160 1163 165.5
18 5.07E-01 0 553.695 182.472 533.454 189.378 486.5 92.5 456 128
19 1.80E-01 0 256.076 156.88 266.75 175.062 170 69 202 102.5
20 -8.45E-02 0 205.353 157.011 216.654 165.156 119 68 140 81

Total number of rows: 14720

Table truncated, full table size 961 Kbytes.




Supplementary data files not provided

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