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Status
Public on May 21, 2004
Title
B chip Version2: time 3 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
121.711
99.8867
180.353
204.1
29
25
104
109
2
NULL
0
159.016
107.358
182.104
174.043
80.5
35
77.5
88
3
2.07E+00
0
3343.91
168.288
1826.1
204.054
3266
92.5
1702
135
4
-1.22E-01
0
174.107
129.92
263.831
198.68
84
53.5
193
109.5
5
6.22E-01
0
1418.12
109.434
1862.69
197.611
1346
33
1639
120
6
4.17E-01
0
425.525
140.875
569.115
177.896
327
55.5
450
111
7
6.90E-01
0
4782.01
162.766
5471.8
221.845
4669.5
75
5422
161.5
8
3.27E-01
0
198.943
117.936
252.68
165.764
104
21
174
73
9
1.64E-01
0
312.737
114.028
480.871
174.46
211
42
379
109
10
-4.12E-01
0
128.947
98.2179
238.249
154.795
62
20
168
72
11
3.11E-02
0
140.799
116.353
193.634
155.763
53
32.5
106
72
12
-2.24E-01
0
122.287
90.6368
212.277
166.658
42
18.5
122.5
74.5
13
5.86E-01
0
404.14
111.362
478.138
163.561
309.5
32.5
423
65
14
-3.84E-01
0
132.607
118.986
211.23
146.366
47
33
132.5
65
15
8.93E-02
0
482.573
123.044
784.266
197.897
386
37.5
674.5
106.5
16
-4.42E-01
0
182.548
131.775
347.113
190.474
88
40
271
116
17
-4.14E-02
0
652.2
140.639
1133.02
201.91
573.5
59
1028.5
129.5
18
-5.07E-02
0
356.294
133.948
617.287
175.174
242
43
518.5
101
19
1.56E-01
0
176.891
125.877
270.297
184.561
73
39
196
120
20
2.69E-01
0
203.834
143.294
229.806
185.481
125
69.5
127
95
Total number of rows: 14720 Table truncated, full table size 951 Kbytes .Supplementary data files not provided