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Sample GSM22844 Query DataSets for GSM22844
Status Public on May 21, 2004
Title B chip Version2: time 3 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 121.711 99.8867 180.353 204.1 29 25 104 109
2 NULL 0 159.016 107.358 182.104 174.043 80.5 35 77.5 88
3 2.07E+00 0 3343.91 168.288 1826.1 204.054 3266 92.5 1702 135
4 -1.22E-01 0 174.107 129.92 263.831 198.68 84 53.5 193 109.5
5 6.22E-01 0 1418.12 109.434 1862.69 197.611 1346 33 1639 120
6 4.17E-01 0 425.525 140.875 569.115 177.896 327 55.5 450 111
7 6.90E-01 0 4782.01 162.766 5471.8 221.845 4669.5 75 5422 161.5
8 3.27E-01 0 198.943 117.936 252.68 165.764 104 21 174 73
9 1.64E-01 0 312.737 114.028 480.871 174.46 211 42 379 109
10 -4.12E-01 0 128.947 98.2179 238.249 154.795 62 20 168 72
11 3.11E-02 0 140.799 116.353 193.634 155.763 53 32.5 106 72
12 -2.24E-01 0 122.287 90.6368 212.277 166.658 42 18.5 122.5 74.5
13 5.86E-01 0 404.14 111.362 478.138 163.561 309.5 32.5 423 65
14 -3.84E-01 0 132.607 118.986 211.23 146.366 47 33 132.5 65
15 8.93E-02 0 482.573 123.044 784.266 197.897 386 37.5 674.5 106.5
16 -4.42E-01 0 182.548 131.775 347.113 190.474 88 40 271 116
17 -4.14E-02 0 652.2 140.639 1133.02 201.91 573.5 59 1028.5 129.5
18 -5.07E-02 0 356.294 133.948 617.287 175.174 242 43 518.5 101
19 1.56E-01 0 176.891 125.877 270.297 184.561 73 39 196 120
20 2.69E-01 0 203.834 143.294 229.806 185.481 125 69.5 127 95

Total number of rows: 14720

Table truncated, full table size 951 Kbytes.




Supplementary data files not provided

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