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Sample GSM22845 Query DataSets for GSM22845
Status Public on May 21, 2004
Title B chip Version2: time 8 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 227.889 214.377 130.65 163.417 141 136 58 91
2 -5.01E-01 0 313.208 261.038 180.1 152.753 266 189 104 54.5
3 7.98E-01 0 2583.7 331.906 847.581 198.765 2443 258 740 125
4 5.57E-01 0 460.615 269.083 223.469 182.738 383 164.5 144 98
5 4.16E-01 0 12085.5 370.372 3959.71 218.43 12351 312 3881 152
6 6.24E-01 0 1442.28 296.671 550.106 198.298 1301 222 475 141
7 -2.73E-01 0 27626.8 473.923 9200.62 306.753 31282 413 9930 210
8 3.24E-01 0 439.889 338.692 235.623 204.968 353 271 149 139
9 5.50E-01 0 538.397 200.657 245.805 165.696 445 107 136 75
10 -1.64E-01 0 257.639 230.258 150.639 150.314 179 150.5 79 56.5
11 2.93E-01 0 240.503 212.139 131.442 143.571 177.5 118 57 52
12 -2.26E-01 0 275.029 211.992 176.027 131.708 179.5 130 99 56.5
13 1.09E+00 0 871.973 203.586 251.521 131.162 740 141.5 148 58.5
14 NULL 0 229.533 207.867 167.518 150.129 163 156 84 75
15 2.24E-01 0 783.903 221.737 381.138 159.108 686 167 291 94
16 -4.55E-01 0 420.683 300.479 250.517 165.042 348 230.5 191 85.5
17 -1.32E-01 0 1218.82 324.243 646.681 204.318 1084 275 566 117
18 4.15E-01 0 960.656 264.954 411.843 179.915 825 182 327 102
19 2.17E-01 0 353.111 261.952 225.652 188.884 276 203 151 131
20 -3.61E-01 0 384.506 260.795 281.916 227.434 308 213 230 136.5

Total number of rows: 14720

Table truncated, full table size 953 Kbytes.




Supplementary data files not provided

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