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Sample GSM22850 Query DataSets for GSM22850
Status Public on May 21, 2004
Title B chip Version2: time 3 day Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 206.781 189.311 179.288 201.746 138.5 120 93.5 110.5
2 NULL 0 245.386 237.302 217.399 176.776 182 173.5 141 112
3 -4.99E-01 0 335.851 221.364 611.159 221.578 276.5 154 513 145.5
4 NULL 0 234.612 195.56 239.791 199.193 153 129 172 143
5 5.84E-01 0 1865.12 264.686 2808.27 217.236 1784 223 2698 164
6 NULL 0 315.08 202.969 368.585 227.459 263 133 295 180
7 4.50E-01 0 6656.91 284.683 10086.3 318.764 7002 190 10617 259
8 NULL 0 256.056 222.922 241.603 214.612 191 159 163 129
9 3.39E-01 0 281.567 189.764 290.289 178.682 208.5 112.5 222.5 106
10 NULL 0 248.009 228.254 199.385 172.589 176.5 166 107.5 111
11 NULL 0 224.865 211.976 195.1 216.436 154 161 116 133
12 NULL 0 241.321 205.831 198.153 186.803 159 142 129 112
13 NULL 0 282.541 271.062 311.142 211.133 220 216 241 143
14 NULL 0 234.45 227.3 191.114 202.245 165 134 110 115
15 -1.45E-01 0 330.06 210.024 453.971 205.638 278 152 380 125
16 2.32E-01 0 279.582 229.569 246.548 179.324 215.5 141 159 107
17 6.00E-02 0 394.843 259.863 496.666 201.236 311 190 420 145.5
18 4.54E-01 0 299.667 232.324 299.996 228.228 248 143 221 158
19 -3.46E-02 0 276.758 243.44 234.572 169.164 196 156.5 147 95.5
20 -3.83E-01 0 284.736 218.851 236.301 171.882 215 178 161 82

Total number of rows: 14720

Table truncated, full table size 938 Kbytes.




Supplementary data files not provided

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