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Sample GSM22851 Query DataSets for GSM22851
Status Public on May 21, 2004
Title B chip Version2: time 3 day Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 194.65 157.648 245.576 249.799 113 88 187 172
2 NULL 0 315.37 190.611 367.475 258.183 227 105 286 175
3 -9.46E-01 0 1547.92 236.724 2460.68 307.917 1280 166.5 1896 231
4 -2.76E-01 0 293.056 200.03 345.019 225.76 225 115 276 154
5 3.60E-01 0 4543.46 234.387 2852.65 289.308 4197 161 2486 227
6 1.37E-02 0 982.182 205.503 952.328 263.213 853 114.5 765 182
7 7.94E-01 0 572.942 200.892 418.73 251.673 455 135 340 192
8 -3.18E-01 0 398.721 221.698 424.541 236.846 288 130.5 330 141
9 1.56E-01 0 738.113 208.447 648.754 234.92 652 134 522 161
10 -3.41E-02 0 295.721 199.21 310.748 238.367 198 109 234 138.5
11 3.12E-01 0 293.582 162.365 314.056 226.64 199 88 252 162
12 2.98E-01 0 246.098 176.475 288.903 253.184 174 98 198 176
13 1.43E-01 0 401.82 195.668 382.562 203.118 326.5 108 274.5 151.5
14 6.51E-03 0 266.63 198.241 290.27 231.818 180 112 227 148
15 1.26E-01 0 980.678 179.642 892.77 235.052 887 103 719 167
16 -2.83E-01 0 393.548 177.634 463.887 227.403 332 105 375 165.5
17 -8.48E-02 0 908.913 230.106 924.652 281.031 765 153 727 205
18 -5.50E-01 0 279.415 245.49 328.027 246.608 195 152 246 160
19 -6.14E-01 0 282.61 208.873 353.257 237.124 207 142.5 253 162
20 2.46E-01 0 274.431 198 296.577 221.459 201 96 220 156.5

Total number of rows: 14720

Table truncated, full table size 954 Kbytes.




Supplementary data files not provided

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