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Status
Public on May 21, 2004
Title
B chip Version2: time 3 day Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
169.941
185.603
181.134
184.934
77
94
94
115
2
5.17E-01
0
265.483
158.066
225.404
180.198
173
66
147
101
3
-8.60E-01
0
679.491
165.041
1036.85
199.37
527
85
867
119
4
-3.53E-01
0
198.357
179.078
225.619
189.954
99
102
146
103
5
3.48E-01
0
2144.81
196.73
1352.18
197.71
2004.5
126
1172
114.5
6
1.27E-02
0
623.928
171.85
536.777
169.59
522
101
418
93
7
1.80E-01
0
531.381
180.567
387.596
173.577
415
104.5
291
71
8
3.31E-01
0
267.346
156.762
267.411
209.294
191.5
58
165.5
122
9
1.75E-01
0
531.075
159.274
448.344
195.406
426.5
87
350
118
10
-1.37E-01
0
217.035
156.708
249.383
204.714
117
79.5
164
109
11
4.55E-01
0
251.867
174.926
222.1
188.599
169
75
127
104
12
4.10E-02
0
192.801
141.298
222.062
166.825
114.5
54.5
155
95
13
2.67E-01
0
281.738
220.45
264.588
209.639
192
141
172
152
14
1.35E-01
0
212.144
154.956
238.363
207.687
116.5
81
161
122.5
15
4.49E-02
0
502.384
174.415
451.515
191.669
373
89
354
115
16
-5.63E-01
0
296.206
181.785
347.5
184.544
221
108.5
271
108
17
-4.10E-01
0
550.536
205.498
650.113
231.058
432
127
541
171
18
2.72E-01
0
236.556
192.104
234
170.114
147
89
148
118
19
-5.83E-01
0
231
169.554
313.404
188.292
141
76.5
243
112
20
-2.45E-01
0
204.986
148.905
278.293
192.659
120.5
59
203.5
122.5
Total number of rows: 14720 Table truncated, full table size 952 Kbytes .Supplementary data files not provided