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Sample GSM22854 Query DataSets for GSM22854
Status Public on May 21, 2004
Title B chip Version2: time 3 day Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 169.941 185.603 181.134 184.934 77 94 94 115
2 5.17E-01 0 265.483 158.066 225.404 180.198 173 66 147 101
3 -8.60E-01 0 679.491 165.041 1036.85 199.37 527 85 867 119
4 -3.53E-01 0 198.357 179.078 225.619 189.954 99 102 146 103
5 3.48E-01 0 2144.81 196.73 1352.18 197.71 2004.5 126 1172 114.5
6 1.27E-02 0 623.928 171.85 536.777 169.59 522 101 418 93
7 1.80E-01 0 531.381 180.567 387.596 173.577 415 104.5 291 71
8 3.31E-01 0 267.346 156.762 267.411 209.294 191.5 58 165.5 122
9 1.75E-01 0 531.075 159.274 448.344 195.406 426.5 87 350 118
10 -1.37E-01 0 217.035 156.708 249.383 204.714 117 79.5 164 109
11 4.55E-01 0 251.867 174.926 222.1 188.599 169 75 127 104
12 4.10E-02 0 192.801 141.298 222.062 166.825 114.5 54.5 155 95
13 2.67E-01 0 281.738 220.45 264.588 209.639 192 141 172 152
14 1.35E-01 0 212.144 154.956 238.363 207.687 116.5 81 161 122.5
15 4.49E-02 0 502.384 174.415 451.515 191.669 373 89 354 115
16 -5.63E-01 0 296.206 181.785 347.5 184.544 221 108.5 271 108
17 -4.10E-01 0 550.536 205.498 650.113 231.058 432 127 541 171
18 2.72E-01 0 236.556 192.104 234 170.114 147 89 148 118
19 -5.83E-01 0 231 169.554 313.404 188.292 141 76.5 243 112
20 -2.45E-01 0 204.986 148.905 278.293 192.659 120.5 59 203.5 122.5

Total number of rows: 14720

Table truncated, full table size 952 Kbytes.




Supplementary data files not provided

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