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Sample GSM22855 Query DataSets for GSM22855
Status Public on May 21, 2004
Title B chip Version2: time 5 day Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 204.022 190.647 128.472 144.425 128 115.5 62 66.5
2 NULL 0 234.05 179.019 150.355 133.433 162 113 73.5 53.5
3 0.641141386 0 962.08 235.537 870.891 146.569 833 152 737 77
4 0.070673453 0 254.357 196.5 191.373 140.752 185 125 126 58.5
5 -0.046983061 0 620.209 214.709 780.266 119.902 411 138 404 57
6 -0.171333005 0 388.635 248.607 344.07 132.357 292.5 167.5 241 51.5
7 -0.125446205 0 5672.58 254.372 8064.69 245.282 5587 192 8426 180
8 NULL 0 219.554 196.321 213.438 174.375 147 133 133 101
9 NULL 0 256.719 221.414 223.759 137.685 190.5 155.5 147 47.5
10 NULL 0 191.989 197.161 138.84 117.376 112 131 61 39
11 NULL 0 209.679 179.553 131.532 116.354 141 91 47 40.5
12 NULL 0 228.854 219.494 180.399 136.669 163 172.5 95 58
13 0.662682099 0 446.116 227.696 288.551 130.673 355.5 168 202 77
14 NULL 0 241.685 198.491 148.115 156.544 184 125 64 66
15 0.226560338 0 454.909 232.346 394.572 148.41 382 149 306 65
16 0.200349666 0 310.228 196.955 269.307 153.006 242 118 190 74.5
17 0.082306857 0 561.628 267.295 613.41 216.383 497 194 524 143.5
18 0.031975785 0 412.087 224.071 400.071 170.006 358 149 321 88
19 NULL 0 230.41 182.131 164.673 158.879 155 109 88 108
20 0.18205596 0 259.723 204.981 171.98 138.444 189 141.5 92 67

Total number of rows: 14720

Table truncated, full table size 965 Kbytes.




Supplementary data files not provided

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