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Status
Public on May 21, 2004
Title
B chip Version2: time 5 day Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
204.022
190.647
128.472
144.425
128
115.5
62
66.5
2
NULL
0
234.05
179.019
150.355
133.433
162
113
73.5
53.5
3
0.641141386
0
962.08
235.537
870.891
146.569
833
152
737
77
4
0.070673453
0
254.357
196.5
191.373
140.752
185
125
126
58.5
5
-0.046983061
0
620.209
214.709
780.266
119.902
411
138
404
57
6
-0.171333005
0
388.635
248.607
344.07
132.357
292.5
167.5
241
51.5
7
-0.125446205
0
5672.58
254.372
8064.69
245.282
5587
192
8426
180
8
NULL
0
219.554
196.321
213.438
174.375
147
133
133
101
9
NULL
0
256.719
221.414
223.759
137.685
190.5
155.5
147
47.5
10
NULL
0
191.989
197.161
138.84
117.376
112
131
61
39
11
NULL
0
209.679
179.553
131.532
116.354
141
91
47
40.5
12
NULL
0
228.854
219.494
180.399
136.669
163
172.5
95
58
13
0.662682099
0
446.116
227.696
288.551
130.673
355.5
168
202
77
14
NULL
0
241.685
198.491
148.115
156.544
184
125
64
66
15
0.226560338
0
454.909
232.346
394.572
148.41
382
149
306
65
16
0.200349666
0
310.228
196.955
269.307
153.006
242
118
190
74.5
17
0.082306857
0
561.628
267.295
613.41
216.383
497
194
524
143.5
18
0.031975785
0
412.087
224.071
400.071
170.006
358
149
321
88
19
NULL
0
230.41
182.131
164.673
158.879
155
109
88
108
20
0.18205596
0
259.723
204.981
171.98
138.444
189
141.5
92
67
Total number of rows: 14720 Table truncated, full table size 965 Kbytes .Supplementary data files not provided