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Status
Public on May 21, 2004
Title
B chip Version2: time 5 day Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
234.453
214.111
153.572
138.494
163
135
80
64.5
2
NULL
0
261.236
226.448
197.233
177.353
172.5
156.5
133.5
101
3
1.00E+00
0
803.237
242.12
650.095
206.479
715
186
564
120
4
-4.54E-01
0
240.469
193.578
203.045
122.88
192
139.5
117
58
5
-3.40E-02
0
1283.52
252.107
2039.94
183.877
1151
193
1946
114
6
-5.00E-01
0
337.384
235.378
360.553
161.73
247.5
179.5
281
93
7
3.04E-02
0
6020.88
247.237
8115.96
253.456
6099
192
8422
183
8
-1.48E-01
0
285.925
255.245
217.887
163.889
228
179.5
131.5
93
9
-5.21E-01
0
270.506
248.549
231.682
158.277
199
166
148
74
10
NULL
0
241.33
235.62
164.021
156.57
174
168.5
81
83.5
11
NULL
0
248.599
218.429
151.347
144.222
182
145
82
85.5
12
3.58E-01
0
261.866
213.277
162.651
136.369
199.5
137
74.5
57
13
4.15E-01
0
453.433
263.457
335.89
143.686
358
189.5
249
71.5
14
NULL
0
225.754
252.891
189.27
128.281
153
180
111
51
15
1.01E-01
0
392.659
207.662
397.932
182.338
329
151
305
100
16
1.66E-01
0
301.388
230.019
267.694
205.773
264
163.5
207
133.5
17
8.72E-02
0
553.882
286.336
625.399
251.82
473
229
512
173.5
18
-5.47E-02
0
441.902
268.963
419.011
171.559
367
216
334
113
19
5.00E-01
0
307.703
249.557
200.211
152.097
234
159
118
84
20
NULL
0
248.441
228.331
183.538
180.624
189
175
107
105
Total number of rows: 14720 Table truncated, full table size 930 Kbytes .Supplementary data files not provided