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Sample GSM22856 Query DataSets for GSM22856
Status Public on May 21, 2004
Title B chip Version2: time 5 day Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 234.453 214.111 153.572 138.494 163 135 80 64.5
2 NULL 0 261.236 226.448 197.233 177.353 172.5 156.5 133.5 101
3 1.00E+00 0 803.237 242.12 650.095 206.479 715 186 564 120
4 -4.54E-01 0 240.469 193.578 203.045 122.88 192 139.5 117 58
5 -3.40E-02 0 1283.52 252.107 2039.94 183.877 1151 193 1946 114
6 -5.00E-01 0 337.384 235.378 360.553 161.73 247.5 179.5 281 93
7 3.04E-02 0 6020.88 247.237 8115.96 253.456 6099 192 8422 183
8 -1.48E-01 0 285.925 255.245 217.887 163.889 228 179.5 131.5 93
9 -5.21E-01 0 270.506 248.549 231.682 158.277 199 166 148 74
10 NULL 0 241.33 235.62 164.021 156.57 174 168.5 81 83.5
11 NULL 0 248.599 218.429 151.347 144.222 182 145 82 85.5
12 3.58E-01 0 261.866 213.277 162.651 136.369 199.5 137 74.5 57
13 4.15E-01 0 453.433 263.457 335.89 143.686 358 189.5 249 71.5
14 NULL 0 225.754 252.891 189.27 128.281 153 180 111 51
15 1.01E-01 0 392.659 207.662 397.932 182.338 329 151 305 100
16 1.66E-01 0 301.388 230.019 267.694 205.773 264 163.5 207 133.5
17 8.72E-02 0 553.882 286.336 625.399 251.82 473 229 512 173.5
18 -5.47E-02 0 441.902 268.963 419.011 171.559 367 216 334 113
19 5.00E-01 0 307.703 249.557 200.211 152.097 234 159 118 84
20 NULL 0 248.441 228.331 183.538 180.624 189 175 107 105

Total number of rows: 14720

Table truncated, full table size 930 Kbytes.




Supplementary data files not provided

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