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Sample GSM22859 Query DataSets for GSM22859
Status Public on May 21, 2004
Title B chip Version2: time 5 day Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 153.412 148.576 227.643 176.76 75 71 159 96
2 -1.01E+00 0 211.578 185.239 333.155 213.607 115 113 260 113
3 2.18E-01 0 1747.87 182.663 2121.16 247.946 1438 118.5 1846 201.5
4 -1.84E-01 0 182.258 165.163 265.318 238.24 94 93.5 177.5 156.5
5 -1.59E-01 0 1042.01 141.764 1527.74 171.375 907 59.5 1273 112
6 6.59E-01 0 803.914 175.823 727.778 211.717 693 93 634 131
7 -2.87E-01 0 284.641 163.23 418.889 220.566 216 88 328 146
8 -4.35E-01 0 233.635 173.613 335.734 190.257 141 90 259 110
9 NULL 0 574.554 165.015 486.164 169.896 470 93 399 92.5
10 -8.05E-01 0 166.361 162.179 264.594 170.29 80.5 81.5 184.5 106
11 9.39E-02 0 191.05 134.245 253.753 186.313 111.5 52.5 186 105
12 NULL 0 198.304 159.738 228.111 160.781 106 60 133 91
13 3.53E-01 0 349.166 180.188 373.118 220.659 283 105 276 150
14 -1.44E-01 0 203.806 171.33 294.994 205.708 127 84 215 147
15 -4.86E-02 0 750 179.101 992.557 208.477 635 91 814 121
16 -8.58E-01 0 326.255 175.333 616.607 204.733 247 100 455 130
17 2.87E-01 0 1038.42 212.259 1170.79 277.71 939 131 898 207
18 5.61E-01 0 358.285 160.854 389.696 223.246 283 86 295 172
19 -4.22E-02 0 305.942 188.022 344.515 174.319 234 99.5 249 100.5
20 -4.08E-01 0 213.497 191.646 279.569 200.258 130.5 105.5 203.5 121.5

Total number of rows: 14720

Table truncated, full table size 942 Kbytes.




Supplementary data files not provided

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