|
Status |
Public on Dec 27, 2007 |
Title |
BP.2 |
Sample type |
RNA |
|
|
Source name |
distal small intestine from Rag 1
|
Organism |
Mus musculus |
Characteristics |
Rag 1-/- mouse with 225.4 anti-B.thetaiotaomicron specific IgA producing hybridoma growing subcutaneously colonized with B. thetaiotaomicron for 10 days.
|
Growth protocol |
Distal Small intestine (Whole intestinal segments 10, 11, 12, and 14 pooled) from mice colonized for 10 days with B. thetaiotaomicron.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
to be added
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 40 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430.2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GS300
|
Description |
Comparison of Wildtype B6 and Rag1 mice colonized for 10 days with Bacteroides thetaiotaomicron
|
Data processing |
Image files were analyzed in dCHIP06, samples were normalized to the B6.2 chip, using the quartile method.
|
|
|
Submission date |
Sep 12, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Daniel A Peterson |
Phone |
314 362 5443
|
Organization name |
Center for Genome Sciences
|
Department |
Molecular Biology and Pharmacology
|
Lab |
Jeffrey I Gordon
|
Street address |
4444 Forest Park
|
City |
St Louis |
State/province |
MO |
ZIP/Postal code |
63021 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE9018 |
IgA impact on distal small intestine after colonization with B. thetaiotaomicron |
|
Relations |
Reanalyzed by |
GSE119085 |