|
Status |
Public on Jan 29, 2008 |
Title |
338_TaqMan |
Sample type |
RNA |
|
|
Source name |
Peripheral blood
|
Organism |
Homo sapiens |
Characteristics |
TAA; training set
|
Extracted molecule |
total RNA |
Extraction protocol |
PAXgene tube and Qiagen Preanalytics Blood RNA kit
|
Label |
FAM
|
Label protocol |
Each TaqMan(R) Assay was run in four replicates for each RNA sample using 10 ng total cDNA (measured as total input RNA) in a 10 ul final volume. Assays were run with 2X Universal Master Mix (without UNG) on Applied Biosystems 7900 Fast Real-Time PCR System using universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
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|
|
Hybridization protocol |
None
|
Scan protocol |
None
|
Description |
Gene expression data from peripheral blood of TAA/normal individuals using TaqMan real-time PCR assays
|
Data processing |
Data was processed using SDS 2.2 software (Applied Biosystems, Foster City, CA)
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|
|
Submission date |
Sep 19, 2007 |
Last update date |
Feb 20, 2008 |
Contact name |
Catalin Barbacioru |
E-mail(s) |
catalin@appliedbiosystems.com
|
Organization name |
Applied Biosystems
|
Street address |
850 Lincoln Centre Dr
|
City |
Foster City |
State/province |
CA |
ZIP/Postal code |
94404 |
Country |
USA |
|
|
Platform ID |
GPL2845 |
Series (1) |
GSE9106 |
Gene Expression Signature in Peripheral Blood Detects Thoracic Aortic Aneurysm |
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