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| Status |
Public on Sep 14, 2017 |
| Title |
control 1 |
| Sample type |
SRA |
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| Source name |
pancreas
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| Organism |
Mus musculus |
| Characteristics |
strain: Mix, C57BL/6, 129SvEv, FvB
tissue: pancreas
age: E17.5
genotype: control (wildtype, NFIA fl/+, NFIA fl/fl)
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| Extracted molecule |
total RNA |
| Extraction protocol |
phenol-chloroform with TRIzol
TruSeq Stranded mRNA kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
NextSeq Control Software v2.0 used for base calling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 using TopHat Alignment v1.0.0
Fragment per kilobase million of library size (FPKM) were calculated using Cufflinks Assembly & DE v1.1.0
Genome_build: RefSeq mm10
Supplementary_files_format_and_content: control_vs_nfia.gene_exp.diff
Supplementary_files_format_and_content: control_vs_nfia.isoform_exp.diff
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| Submission date |
Sep 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Malgorzata Borowiak |
| E-mail(s) |
borowiak@bcm.edu
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| Organization name |
Baylor College of Medicine
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| Street address |
1 Baylor Plaza
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| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platform ID |
GPL19057 |
| Series (1) |
| GSE86909 |
NFIA regulates pancreatic cell fate and adult physiology through vesicle trafficking |
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| Relations |
| BioSample |
SAMN05771112 |
| SRA |
SRX2163104 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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