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Sample GSM2332115 Query DataSets for GSM2332115
Status Public on Dec 01, 2018
Title CO1_390209M
Sample type RNA
 
Source name tissue biopsy
Organism Homo sapiens
Characteristics subject: CO1_390209M
diseaseextent: --
disease: Normal
age: --
tissue: Colon
Treatment protocol Mucosal biopsies were collected 15 to 20 cm from the anal verge at screening during endoscopies from a subset of patients who participated in the PURSUIT (n=87) study. Independent of the PURSUIT study , biopsies were also collected from 21 healthy normal subjects to serve as control. Biopsies were immediately placed in RNAlater and subsequently stored at –80oC until RNA preparation.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
Label biotin
Label protocol Target was prepared from 100ng total RNA using the NuGEN Ovation V2 kit, followed by NuGEN Encore biotin labeling module, using the manufacturer’s protocol.
 
Hybridization protocol Following fragmentation, 1.875ug of cDNA was hybridized for 16 hr at 45oC on GeneChip HT HG-U133+ PM 96 array plates. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
Scan protocol GeneChips were scanned using the GeneChipHT Scanner.
Data processing The data was normalized by RMA using ArrayStudio version 9.
 
Submission date Sep 29, 2016
Last update date Dec 01, 2018
Contact name K Li
E-mail(s) kli@its.jnj.com
Organization name Janssen R&D
Street address 1400 McKean Road
City Spring House
State/province PA
ZIP/Postal code 19477
Country USA
 
Platform ID GPL13158
Series (2)
GSE87466 Gene expression profiling of mucosal biopsies from adult patients with moderately to severely active ulcerative colitis
GSE87473 Gene expression profiling of mucosal biopsies from pediatric or adult patients with moderately to severely active ulcerative colitis

Data table header descriptions
ID_REF
VALUE RMA normalized intensity in log2 transformation.

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.4168
AFFX-BioB-M_at 7.2774
AFFX-BioB-3_at 7.0339
AFFX-BioC-5_at 10.8073
AFFX-BioC-3_at 9.9224
AFFX-BioDn-5_at 11.9422
AFFX-BioDn-3_at 12.7976
AFFX-CreX-5_at 13.6626
AFFX-CreX-3_at 13.9771
AFFX-DapX-5_at 3.883
AFFX-DapX-M_at 3.2535
AFFX-DapX-3_at 3.3394
AFFX-LysX-5_at 4.0115
AFFX-LysX-M_at 3.9971
AFFX-LysX-3_at 3.253
AFFX-PheX-5_at 4.3182
AFFX-PheX-M_at 3.6353
AFFX-PheX-3_at 5.1222
AFFX-ThrX-5_at 3.6705
AFFX-ThrX-M_at 3.884

Total number of rows: 54715

Table truncated, full table size 1109 Kbytes.




Supplementary file Size Download File type/resource
GSM2332115_5500254131236090112259_101136.C11.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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