|
| Status |
Public on Dec 01, 2018 |
| Title |
E5687747-5 |
| Sample type |
RNA |
| |
|
| Source name |
tissue biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
subject: 7460-00004
diseaseextent: Limited
disease: Ulcerative Colitis (UC)
age: 45
tissue: Colon
|
| Treatment protocol |
Mucosal biopsies were collected 15 to 20 cm from the anal verge at screening during endoscopies from a subset of patients who participated in the PURSUIT (n=87) study. Independent of the PURSUIT study , biopsies were also collected from 21 healthy normal subjects to serve as control. Biopsies were immediately placed in RNAlater and subsequently stored at –80oC until RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Target was prepared from 100ng total RNA using the NuGEN Ovation V2 kit, followed by NuGEN Encore biotin labeling module, using the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.875ug of cDNA was hybridized for 16 hr at 45oC on GeneChip HT HG-U133+ PM 96 array plates. GeneChips were washed and stained in the Affymetrix GeneChip Array Station.
|
| Scan protocol |
GeneChips were scanned using the GeneChipHT Scanner.
|
| Data processing |
The data was normalized by RMA using ArrayStudio version 9.
|
| |
|
| Submission date |
Sep 29, 2016 |
| Last update date |
Dec 01, 2018 |
| Contact name |
K Li |
| E-mail(s) |
kli@its.jnj.com
|
| Organization name |
Janssen R&D
|
| Street address |
1400 McKean Road
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE87466 |
Gene expression profiling of mucosal biopsies from adult patients with moderately to severely active ulcerative colitis |
| GSE87473 |
Gene expression profiling of mucosal biopsies from pediatric or adult patients with moderately to severely active ulcerative colitis |
|
