|
| Status |
Public on Oct 12, 2016 |
| Title |
ABS-33 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Autism Spectrum Disorder
disease state: inflamed
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from biopsy tissues by sonicating in the presence of TriReagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy MinelutePlus mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyzer. For RNA isolation from blood, we used a QIAcube robotic workstation with RNeasyPlus kits following the manufacturer's protocols. As before, RNA quality was assessed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
9970518039_I
|
| Data processing |
The data were normalized using quantile normalization in Genome Studio
|
| |
|
| Submission date |
Oct 11, 2016 |
| Last update date |
Oct 12, 2016 |
| Contact name |
Stephen Walker |
| E-mail(s) |
swalker@wakehealth.edu
|
| Organization name |
Wake Forest University Health Sciences
|
| Street address |
Medical Center Blvd
|
| City |
Winston Salem |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
|
| Platform ID |
GPL18281 |
| Series (1) |
| GSE87847 |
A Putative Blood-Based Biomarker for Autism Spectrum Disorder-Associated Ileocolitis |
|
