|
| Status |
Public on Nov 15, 2016 |
| Title |
CordBlood preDCs-CD172a- Rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Cord Blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Cord Blood
cell type: preDCs-CD172a-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and column-purified using the Arcturus PicoPure RNA Isolation kit (Applied Biosystems). Genomic DNA was removed by on-column digest with DNAse I (Qiagen), according to the PicoPure RNA Isolation kit manual
RNA libraries were prepared using the SMARTer Ultra Low Input RNA for Sequencing kit (Clontech Laboratories) followed with Nextera XT DNA Library Prep kit (Illumina). Libraries were sequenced by 75-bp single-end reading on a NextSeq 500 sequencer (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
alignment with STAR 2.4.1d (default settings)
Gene expression counts generated with htseq-count version 0.6.0
Genome_build: hg19
Supplementary_files_format_and_content: the processed data files includes the refGene gene symbol and counts
|
| |
|
| Submission date |
Oct 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Israel Tojal da Silva |
| E-mail(s) |
itojal@cipe.accamargo.org.br
|
| Phone |
1121895000
|
| Organization name |
HOSPITAL A C CAMARGO
|
| Street address |
Tagua, 440
|
| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
01508-010 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE88858 |
RNA-Seq gene profiling comparison from human cDC subsets and pre-cDC subsets |
|
| Relations |
| BioSample |
SAMN05915869 |
| SRA |
SRX2250299 |
