|
| Status |
Public on Aug 30, 2017 |
| Title |
daf-16_fasting |
| Sample type |
RNA |
| |
|
| Source name |
daf-16_fasting
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
age: day 4 adult
strain: daf-16(mu86)
condition: fasting
|
| Treatment protocol |
The day on which the animals were transferred to FUDR-containing NGM plates was defined as t = 0 day. On day 2, the animals were transferred to FUDR-containing NGM plates that were seeded with or without UV-killed OP50.
|
| Growth protocol |
All strains were maintained at 20 °C on nematode growth medium (NGM) plates seeded with Escherichia coli OP50.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
3` IVT
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip C. elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from daf-16 mutant in the fasting conditions.
|
| Data processing |
genespring GX
|
| |
|
| Submission date |
Nov 07, 2016 |
| Last update date |
Aug 30, 2017 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL200 |
| Series (2) |
| GSE89614 |
Role of the MicroRNA Machinery in Fasting-Induced Gene Expression Changes and Longevity in C. elegans (mRNA) |
| GSE89624 |
Role of the MicroRNA Machinery in Fasting-Induced Gene Expression Changes and Longevity in C. elegans |
|
