|
| Status |
Public on Jan 08, 2017 |
| Title |
Identification of miR-150-3p target genes in A549 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mock
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: lung adenocarcinoma
transfection: no transfection
|
| Treatment protocol |
microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
|
| Growth protocol |
Normal cell culture medium RPMI 1640 or DMEM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Channel 2 |
| Source name |
miR-150-3p transfected cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: lung adenocarcinoma
transfection: miR-150-3p
|
| Treatment protocol |
microRNA or siRNA transfection using Lipofectamine RNAiMAX (Invitrogen).
|
| Growth protocol |
Normal cell culture medium RPMI 1640 or DMEM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution. Images were quantified using Agilent Feature Extraction software version 10.7.3.1
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jan 07, 2017 |
| Last update date |
Jan 08, 2017 |
| Contact name |
Naohiko Seki |
| E-mail(s) |
naoseki@faculty.chiba-u.jp
|
| Phone |
+81-43-226-2971
|
| Organization name |
Chiba University
|
| Department |
Functional Genomics
|
| Street address |
1-8-1, Inohana
|
| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20844 |
| Series (1) |
| GSE93290 |
Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines (A-498, 786-O, A549, TE-8, PANC-1, SW1990, PC3 and PC3M) |
|
