|
| Status |
Public on Jun 18, 2018 |
| Title |
Patient 5_Neuroendocrine tissue |
| Sample type |
RNA |
| |
|
| Source name |
pituitary tissue_macroadenoma
|
| Organism |
Homo sapiens |
| Characteristics |
pathology: Cushing's disease
patient id: Patient 5
age: 69
size of the tumor: macroadenoma
tissue: ACTH-secreting pituitary adenomas
storage: formalin-fixed paraffin-embedded
usp8 variant status: USP8 wildtype
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Recover All Total Nucleic Acid isolation specific for FFPE tissues (Life Technologies) in accordance with the kit protocol. RNA quantity was detected using IMPLEN NanoPhotometer, while RNA quality measuring gene expression of RPL13A as suggested in literature.
|
| Label |
biotinylated- primers
|
| Label protocol |
Biotinylated cDNA were obtained using biotinylated primers in the reverse transcription reaction according the WG-DASL HT Assay (Illumina protocol)
|
| |
|
| Hybridization protocol |
Biotinylated cDNA is annealed to assay oligonucleotides and bound to streptavidin conjugated paramagnetic particles. After oligo hybridization, mis-hybridized and non-hybridized oligo are washed away.(Illumina WG-DASL HT Assay protocol)
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Patient 5
|
| Data processing |
Data matrix was obtained from 40 patients and 20818 genes, AVG signal was normalized using quantile normalization
Data normalization was obtained using Genome Studio Software (Illumina)
|
| |
|
| Submission date |
Jan 19, 2017 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Francesca Pecori Giraldi |
| Organization name |
Istituto Auxologico Italiano
|
| Department |
Neuroendocrinology Research Laboratory
|
| Street address |
via Zucchi 18
|
| City |
Cusano Milanino |
| State/province |
Milan |
| ZIP/Postal code |
20095 |
| Country |
Italy |
| |
|
| Platform ID |
GPL18281 |
| Series (1) |
| GSE93825 |
Gene expression profiling in human ACTH-secreting pituitary tumors |
|
