|
Status |
Public on Jan 16, 2018 |
Title |
CQ11_Mo_Gene_ST_2_11_SIRT6KD_T0_Un2 |
Sample type |
RNA |
|
|
Source name |
CQ1111_SIRT6KD_T0_Un2
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: SIRT6KD time point: T0 treatment: untreated
|
Treatment protocol |
Two types of mouse(Wt vs. SIRT6) were treated with Un-untreated vs Tr-treated of inhibitor under three time points.
|
Growth protocol |
Mouse grow in NIDDK animal core facility.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Data processing |
The data were nomanized by RMA with Parteck Genomic Suit 6.6
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|
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Submission date |
Jan 25, 2017 |
Last update date |
Jan 19, 2018 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE94058 |
SIRT6 is Essential for Adipocyte Differentiation through Regulating Mitotic Clonal Expansion |
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