ISOGEN® (NIPPON GENE, Tokyo, Japan) extraction of total RNA was performed according to the manufacturer's instructions. The total RNA was analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany)
Label
Cy5
Label protocol
see CodeLink application notes and manuals
Hybridization protocol
see CodeLink application notes and manuals
Scan protocol
GeneChips were scanned using the GenePix® 4000B (Molecular Devices, Sunnyvale, CA, USA) and converted to numerical data with CodeLinkTM Expression Analysis v5.0 (Applied Microarrays, Tempe, AZ, USA).
Description
primary SMZL cells at the different clinical periods (aggressive phases)
Data processing
The analysis was performed using the MicroArray Data Analysis Tool Ver3.2 (Filgen, Aichi, Japan). A log base 2 transformation was applied to the microarray raw data, and global normalization was used to median over entire array.
