|
Status |
Public on Dec 17, 2007 |
Title |
striatum_wildtype_63 |
Sample type |
RNA |
|
|
Source name |
Striatum (brain) from wild-type mice
|
Organism |
Mus musculus |
Characteristics |
Strain: C57Bl/6 / CBA; Gender: female; Age: 85 days; Tissue: striatum (brain); Mouse model: wild-type
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol (Invitrogen) followed by RNeasy column cleanup (Qiagen) using the manufacturers’ protocols
|
Label |
Biotin
|
Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual)
|
|
|
Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip® Expression Analysis Technical Manual)
|
Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual)
|
Description |
Striatal sample from a 12-week-old wild-type mouse.
|
Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
|
|
Submission date |
Dec 06, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Alexandre Kuhn |
E-mail(s) |
kuhnam@mail.nih.gov
|
Organization name |
NIH
|
Department |
NIA
|
Lab |
Laboratory of Neurogenetics
|
Street address |
35 Convent Dr
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892-3707 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (3) |
GSE9803 |
Striatal gene expression data from 12 weeks-old R6/2 mice and control mice (set 1) |
GSE9857 |
Striatal gene expression data from 12 weeks-old R6/2 mice and control mice |
GSE10263 |
Mutant huntingtin's effects on striatal gene expression in mice |
|
Relations |
Reanalyzed by |
GSE119085 |