|
| Status |
Public on Feb 18, 2018 |
| Title |
BRAH099 |
| Sample type |
RNA |
| |
|
| Source name |
BRAH099
|
| Organism |
Homo sapiens |
| Characteristics |
cohort: BRAH cohort
outcome at 6 months: Alive
tissue: liver
|
| Treatment protocol |
na
|
| Growth protocol |
na
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using AllPrepDNA/RNA mini kit (Qiagen) according to manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Refer to the NanoString nCounter Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the NanoString nCounter Assay System Manual
|
| Scan protocol |
Refer to the NanoString nCounter Assay System Manual
|
| Data processing |
For each probe, transcript count data were log2 transformed and scaled by geometric mean of the normalization genes by NanoString normalizer module of GenePattern toolkit (www.broadinstitute.org/genepattern) after setting a floor value of 1.1.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL23098 |
| Series (2) |
| GSE94417 |
An integrative transcriptomic and clinical score for mortality prediction in severe alcoholic hepatitis treated with corticosteroids |
| GSE95246 |
Prognostic liver signature profiles (114-gene signature) in liver biopsy tissues from severe alcoholic hepatitis patients (NanoString) |
|
