|
| Status |
Public on Jan 02, 2018 |
| Title |
colorectal cancer sample AUS2_543 |
| Sample type |
RNA |
| |
|
| Source name |
Colorectal cancer patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colorectal cancer biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from colorectal cancer tissue samples using the Qiagen Allprep DNA/RNA/miRNA Universal kit, according to the manufacturer's protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and labelled according to the Affymetrix GeneChip HT WT User Guide.
|
| |
|
| Hybridization protocol |
Samples were hybridized onto Affymetrix Human Transcriptome 2.0 Arrays according to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's protocol (Affymetrix).
|
| Description |
SST RMA expression values at the gene-level derived using Expression Console software
|
| Data processing |
Gene-level expression signals were obtained from raw data CEL files by the SST RMA procedure implemented in the Affymetrix Expression Console software.
|
| |
|
| Submission date |
Mar 13, 2017 |
| Last update date |
Jan 02, 2018 |
| Contact name |
Anita Sveen |
| E-mail(s) |
anita.sveen@rr-research.no
|
| Organization name |
Institute for Cancer Research, Oslo University Hospital
|
| Department |
Department of Molecular Oncology
|
| Street address |
P.O.Box 4953, Nydalen
|
| City |
Oslo |
| ZIP/Postal code |
NO-0424 |
| Country |
Norway |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE96528 |
Gene expression profiling of colorectal cancer tissue samples. |
|
