|
Status |
Public on Mar 23, 2017 |
Title |
miR-NC C |
Sample type |
RNA |
|
|
Source name |
Supraclavicular Lymph Node
|
Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP transfection: miR-NC
|
Treatment protocol |
Transient transfection for 24 hours with 30nM miRNA with Lipofectamine 2000
|
Growth protocol |
RPMI supplemented with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Modified Trizol protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Androgen Sensitive cell line
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Mar 22, 2017 |
Last update date |
Mar 23, 2017 |
Contact name |
Peter Leedman |
E-mail(s) |
kirsty.richardson@perkins.uwa.edu.au
|
Organization name |
Harry Perkins Institute of Medical Research
|
Street address |
6 Verdun Street
|
City |
Nedlands |
ZIP/Postal code |
6009 |
Country |
Australia |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE96918 |
miR-NC or miR-331-3p treatment of LNCaP prostate cancer cells |
|