|
| Status |
Public on Mar 23, 2017 |
| Title |
miR-331-3p B |
| Sample type |
RNA |
| |
|
| Source name |
Supraclavicular Lymph Node
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
transfection: miR-331-3p
|
| Treatment protocol |
Transient transfection for 24 hours with 30nM miRNA with Lipofectamine 2000
|
| Growth protocol |
RPMI supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Modified Trizol protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Androgen Sensitive cell line
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 22, 2017 |
| Last update date |
Mar 23, 2017 |
| Contact name |
Peter Leedman |
| E-mail(s) |
kirsty.richardson@perkins.uwa.edu.au
|
| Organization name |
Harry Perkins Institute of Medical Research
|
| Street address |
6 Verdun Street
|
| City |
Nedlands |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE96918 |
miR-NC or miR-331-3p treatment of LNCaP prostate cancer cells |
|
